Methane → Kanamycin

HRID 1605807

Reaction equation

[CH2:1]1[C@H:6]([NH2:7])[C@@H:5]([O:8][C@H:9]2[O:14][C@H:13]([CH2:15][NH2:16])[C@@H:12]([OH:17])[C@H:11]([OH:18])[C@H:10]2[OH:19])[C@H:4]([OH:20])[C@@H:3]([O:21][C@H:22]2[O:27][C@H:26]([CH2:28][OH:29])[C@@H:25]([OH:30])[C@H:24]([NH2:31])[C@H:23]2[OH:32])[C@@H:2]1[NH2:33].OS(O)(=O)=O.C>O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>[CH2:1]1[C@H:6]([NH2:7])[C@@H:5]([O:8][C@H:9]2[O:14][C@H:13]([CH2:15][NH2:16])[C@@H:12]([OH:17])[C@H:11]([OH:18])[C@H:10]2[OH:19])[C@H:4]([OH:20])[C@@H:3]([O:21][C@H:22]2[O:27][C@H:26]([CH2:28][OH:29])[C@@H:25]([OH:30])[C@H:24]([NH2:31])[C@H:23]2[OH:32])[C@@H:2]1[NH2:33]

Participants

Conditions

Temperature: 120 °C

Procedure

Simulated kanamycin culture liquid is prepared by adding 80 mg of kanamycin sulfate to 160 ml of glucose-bouillon medium which is sterilized by heating at 120° C for 15 minutes. This liquid is then passed through a column (diameter: 1.5 cm), wherein 2.7 g of the molecular sieving particle containing activated charcoal obtained in Example 1 (sample (a)) is packed, at a flow rate of 0.5 ml/minute. The column is then subjected to washing with water and further to elution with 0.1N HCl-methanol (1:1) solvent. Active fractions are combined and 72.8 mg of kanamycin chloride is recovered in very high purity (decoloration degree: 99%; purity: 98%) and high yield (recovery percentage: 91%).

Workup

washto washing with water and further to elution with 0.1N HCl-methanol (1:1) solvent
custom72.8 mg of kanamycin chloride is recovered in very high purity (decoloration degree: 99%; purity: 98%)
customhigh yield (recovery percentage: 91%)

Sources & evidence

ORD record: ord-e8697ca2080449b0bf6abd3571ce8c7c
Source dataset: uspto-grants-1976_04
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US03951859

Linked notes

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