Glycerol → Bistris

HRID 1673567Notes

Reaction equation

HRID 1673567 reaction equation
CCCCCCCCCC[CH2:11][CH2:12][O:13]S([O-])(=O)=O.[Na+].[OH:19][CH2:20][CH:21](CO)O.C(S)[C@@H](O)[C@H](O)CS.C1C=CC2S(=O)(=O)OC(C3C=C(Br)C(O)=C(Br)C=3)(C3C=C(Br)C(O)=C(Br)C=3)C=2C=1.[CH2:62]([OH:69])[C:63]([NH2:68])([CH2:66][OH:67])[CH2:64][OH:65]>>[CH2:21]([N:68]([C:63]([CH2:66][OH:67])([CH2:64][OH:65])[CH2:62][OH:69])[CH2:11][CH2:12][OH:13])[CH2:20][OH:19]

Participants

6

Reactant 5

HCID 753GlycerolHCID 6503Tris(hydroxymethyl)aminomethaneHCID 8272Bromophenol BlueHCID 446094D-1,4-DithiothreitolHCID 3423265Sodium Lauryl Sulfate2×

Product 1

HCID 81462BistrisYield 10%

Procedure

Protein samples were prepared for SDS-PAGE by diluting 1:1 with 2× denaturing buffer (40 μg/ml SDS, 20% glycerol, 30 μg/ml DTT and 10 μg/ml bromophenol blue in 125 mM Tris, pH 6.8), and boiling for 2 min. Pre-cast Nu-PAGE 10% Bis-Tris protein gels were obtained from Invitrogen (Carlsbad, Calif.). Each lane was loaded with a maximum of 10 μl of sample, and run for 50 min at 200V. After electrophoresis was complete, the gel was stained with Invitrogen SafeStain (1 hour in staining solution, then washed overnight with water).

Sources & evidence

ORD record
ord-3f04a97701c145e6bd734766b4a10cec
Source dataset
uspto-grants-2011_08
Patent number
US08000904B2

Reaction notes

Loading…