Reaction equation
C1[N:6](CCO)[CH2:5][CH2:4][N:3]([CH2:10]CS(O)(=O)=O)C1.[Na+].[Cl-].C[N+](CCSS(C)(=O)=[O:26])(C)C.[Br-].NO.CCCCCCN=C(N=C(N)N)N.C(S)[C@@H](O)[C@H](O)CS.C([N:56]([C:60]([CH2:65]O)([CH2:63][OH:64])CO)CCO)CO>>[NH2:56][C@H:60]([C:63]([OH:26])=[O:64])[CH2:65][C:4]1[N:3]=[CH:10][NH:6][CH:5]=1Participants
8
Reactant 7
Product 1
Procedure
Reactions were assembled in a volume of 260 μl containing 50 mM Hepes buffer, 150 mM NaCl, pH 7.5 and as indicated 5 μM SrtADN in 50 mM BisTris, pH 7.5, 10 μM LPXTG peptide (DABCYL-QALPETGEE-EDANS), 10 μM TGXLP peptide (DABCYL-QATGELPEE-EDANS), 5 mM MTSET, 0.2 M NH2OH, 5 mM pHMB or 10 mM DTT. Incubations were carried out for 1 hour at 37° C. Samples were analyzed in a fluorimeter using 395 nm for excitation and 495 nm for recordings.
Sources & evidence
- ORD record
- ord-74f5c15cb2534009a185745b826cea0d
- Source dataset
- uspto-grants-2006_09
- Patent number
- US07101692B2
Linked notes
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