4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid → Squalene

HRID 258140

Reaction equation

C1N(CCO)CCN([CH2:10][CH2:11]S(O)(=O)=O)C1.[F-].[K+].[CH2:18](S)[C@@H:19](O)[C@H:20](O)[CH2:21]S>>[CH3:18][C:19](=[CH:20][CH2:21][CH2:18]/[C:19](=[CH:20]/[CH2:21][CH2:10]/[C:11](=[CH:10]/[CH2:11][CH2:18]/[CH:19]=[C:20](/[CH2:21][CH2:18]/[CH:19]=[C:20](/[CH2:10][CH2:11][CH:10]=[C:10]([CH3:11])[CH3:11])\[CH3:21])\[CH3:21])/[CH3:20])/[CH3:19])[CH3:18]

Participants

Reactant 3

HCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid2×

HCID 446094D-1,4-Dithiothreitol

HCID 522689Potassium Fluoride2×

Product 1

HCID 638072Squalene

Procedure

Buffer mixture contains 270 mM HEPES, pH 7.5, 20 mM Potassium fluoride and 5.4 mM Dithiothreitol(DTT). 55 μl of this mixture was used per assay. The final concentrations of HEPES, KF and DTT in the assay are 150 mM, 11 mM and 3 mM respectively.

Sources & evidence

ORD record: ord-b6b2a7cdd38d472a9e46f5d143d3e42e
Source dataset: uspto-grants-1993_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05264593

Linked notes

Loading…