Calcium Carbonate → L-Tryptophan

HRID 268591

Reaction equation

HRID 268591 reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])O)O)O)O.S([O-])([O-])(=O)=O.[NH4+:18].[NH4+].P([O-])([O-])(O)=O.[K+].[K+].P([O-])(O)(O)=O.[K+].[OH2:33].O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].OC(CCC[CH2:52][C@H:53]1[C@@H:61]2[C@@H:56]([NH:57][C:58](N2)=O)CS1)=O.C(=O)([O-])[O-].[Ca+2]>O.O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Mn+2]>[NH2:18][C@H:9]([C:11]([OH:12])=[O:33])[CH2:7][C:5]1[C:3]2[C:56](=[CH:61][CH:53]=[CH:52][CH:2]=2)[NH:57][CH:58]=1

Participants

10

Reactant 7

HCID 10112Calcium CarbonateHCID 24450Potassium Phosphate, DibasicHCID 24843Magnesium Sulfate HeptahydrateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×HCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 6097028Ammonium Sulfate

Product 1

HCID 6305L-Tryptophan

Reagents, catalysts & solvents 2

HCID 166832Sulfuric acid, manganese(2+) salt (1:1), heptahydrateCatalystHCID 962WaterSolvent

Procedure

Each of the strain BPL-31 and its parent strain FERM BP-1777 was cultured at 30° C. for 24 hours on the BYG agar medium and then inoculated into a test tube charged with 6 ml of a seed medium (a medium prepared by dissolving 20 g of glucose, 15 g of peptone, 15 g of yeast extract, 2.5 g of sodium chloride, 1 g of urea, 200 mg of L-phenylalanine and 200 mg of L-tyrosine in 1 liter of water and adjusting its pH to 7.2) and cultured at 30° C. for 24 hours. Next, 2 ml of the seed culture was inoculated into a 300 ml capacity conical flask charged with 20 ml of a main culture (a medium prepared by dissolving 60 g of glucose, 10 g of corn steep liquor, 20 g of ammonium sulfate, 0.5 g of dipotassium hydrogenphosphate, 0.5 g of potassium dihydrogenphosphate, 0.25 g of magnesium sulfate heptahydrate, 10 mg of manganese sulfate heptahydrate and 0.03 g of biotin in 1 liter of water, adjusting its pH to 7.2 and then adding 20 g of calcium carbonate thereto) and cultured at 30° C. for 72 hours.

Workup

  1. custominoculated into a test tube
  2. additioncharged with 6 ml of a seed medium (a medium
  3. customprepared
  4. extractionextract
  5. additioncharged with 20 ml of a main culture (a medium
  6. customprepared
  7. wait) and cultured at 30° C. for 72 hours

Sources & evidence

ORD record
ord-94fbbf596b02432db58573d3c89ea4bc
Source dataset
uspto-grants-2008_11
Patent number
US07445915B2

Linked notes

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