Diethyl Ether → 2'-Deoxy-5'-o-phthalimidouridine

HRID 323530

Reaction equation

HRID 323530 reaction equation
[Si]([O:8][CH2:9][C@H:10]1[O:14][C@@H:13]([N:15]2[CH:23]=[C:21](C)[C:19](=[O:20])[NH:18][C:16]2=[O:17])[CH2:12][C@@H:11]1[OH:24])(C(C)(C)C)(C)C.C1(P(C2C=CC=CC=2)C2C=CC=CC=2)C=CC=CC=1.O[N:45]1[C:49](=[O:50])[C:48]2=[CH:51][CH:52]=[CH:53][CH:54]=[C:47]2[C:46]1=[O:55].CCOCC>C1COCC1>[C:49]1(=[O:50])[N:45]([O:8][CH2:9][C@H:10]2[O:14][C@@H:13]([N:15]3[CH:23]=[CH:21][C:19](=[O:20])[NH:18][C:16]3=[O:17])[CH2:12][C@@H:11]2[OH:24])[C:46](=[O:55])[C:47]2=[CH:54][CH:53]=[CH:52][CH:51]=[C:48]12

Participants

6

Reactant 4

HCID 3283Diethyl Ether2×HCID 10665N-HydroxyphthalimideHCID 11776TriphenylphosphineHCID 102512595'-O-tert-Butyldimethylsilylthymidine

Product 1

HCID 103172142'-Deoxy-5'-o-phthalimidouridineYield 62%

Reagents, catalysts & solvents 1

HCID 8028TetrahydrofuranSolvent

Conditions

Temperature
0 °C

Procedure

To a solution of 5′-O-t-butyldimethylsilylthymidine (1, 21.36 g, 60 mmol, prepared according to the procedure of Nair, et al., Org. Prep. Procedures Int. 1990, 22, 57 in dry THF (750 ml)), triphenylphosphine (17.28 g, 66 mmol) and N-hydroxyphthalimide (10.74 g, 66 mmol) were added. The solution was cooled to 0° C. and diisoprbpylazodicarboxylate (15.15 g, 75 mmol) was added dropwise over a period of 3 hr while stirring under nitrogen. The reaction mixture was then stirred at room temperature for 12 hr. The solution was evaporated and the residue was dissolved in CH2Cl2 (750 ml), extracted with sat. NaHCO3 (200 ml), and water (200 ml), dried (MgSO4), filtered and concentrated to furnish yellow oily residue. Silica gel column chromatography (100% hexanes, and then hexanes:Et2O gradient to 90% Et2O) of the residue gave compound 2 as a colorless glass (18.68 g, 62%); 1H NMR (CDCl3) δ 0.05 [2s, 6, (CH3)2], 0.91 [s, 9, (CH3)3], 2.0 (s, 3, CH3), 2.5-2.65 (m, 2, 2′CH2), 4.05-4.2 (m, 2, 5′CH2), 4.25-4.35 (m, 1, 4′H), 5.0 (m, 1, 3′H), 6.15 (m, 1, 1′H), 8.6 (br s, 1, NH), and aromatic protons. Anal. Calcd. for C24H31N3O7Si: C, 57.46;H, 6.23; N, 8.37. found : C, 57.20; H, 6.26; N, 8.27.

Workup

  1. additiondiisoprbpylazodicarboxylate (15.15 g, 75 mmol) was added dropwise over a period of 3 hr
  2. stirringThe reaction mixture was then stirred at room temperature for 12 hr
  3. customThe solution was evaporated
  4. dissolutionthe residue was dissolved in CH2Cl2 (750 ml)
  5. extractionextracted with sat. NaHCO3 (200 ml), and water (200 ml)
  6. dry with materialdried (MgSO4)
  7. filtrationfiltered
  8. concentrationconcentrated
  9. customto furnish yellow oily residue

Sources & evidence

ORD record
ord-b782023a5cea4e0eaf6b72d6c9c8a909
Source dataset
uspto-grants-2001_04
Patent number
US06214551B1

Linked notes

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