Alendronic Acid → HCID 87302179, compound

HRID 39266

Reaction equation

HRID 39266 reaction equation
[CH2:1](CN)[CH2:2][C:3]([P:9]([OH:12])(O)=O)([P:5](O)(O)=[O:6])O.CCN([CH:21]([CH3:23])[CH3:22])C(C)C.CN(C(ON1N=NC2C=C[CH:37]=[CH:38][C:33]1=2)=[N+](C)C)C.F[P-](F)(F)(F)(F)F.[NH2:48][CH2:49][C:50]([NH:52][C@H:53]([C:61]([NH:63][CH2:64][C:65]([OH:67])=[O:66])=[O:62])[CH2:54][C:55]1[CH:60]=[CH:59][CH:58]=[CH:57][CH:56]=1)=[O:51].CN([CH:71]=[O:72])C>C(OCC)(=O)C>[CH2:61]([P:5]([CH:3]([P:9]([CH2:23][CH:21]=[CH2:22])([CH2:33][CH:38]=[CH2:37])=[O:12])[CH2:2][CH2:1][C:71]([NH:48][CH2:49][C:50]([NH:52][C@H:53]([C:61]([NH:63][CH2:64][C:65]([OH:67])=[O:66])=[O:62])[CH2:54][C:55]1[CH:60]=[CH:59][CH:58]=[CH:57][CH:56]=1)=[O:51])=[O:72])([CH2:60][CH:55]=[CH2:56])=[O:6])[CH:53]=[CH2:54]

Participants

7

Reactant 5

HCID 2088Alendronic AcidHCID 6228DimethylformamideHCID 81531DiisopropylethylamineHCID 2733084Methanaminium, N-((1H-benzotriazol-1-yloxy)(dimethylamino)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1)HCID 6992441Glycyl-phenylalanyl-glycine

Product 1

HCID 87302179HCID 87302179, compound

Reagents, catalysts & solvents 1

HCID 8857Ethyl AcetateSolvent

Conditions

Temperature
0 °C

Procedure

To a solution of bisphosphonate 8 (400 mg, 0.98 mmol) in DMF (5 mL) at 0° C. was added DIEA (341 μL, 1.96 mmol) and HBTU (372 mg, 0.98 mmol). After stirring for 10 min at 0° C., the resulting activated ester solution was added to crude tripeptide 75 (0.98 mmol) and stirring was pursued for 2.5 h at 0° C. The reaction mixture was diluted with ethyl acetate, washed with H2O, 1N HCl solution, saturated NaHCO3 solution and saturated NaCl solution. The organic layer was dried over MgSO4, filtered and concentrated to dryness. The crude product was purified by silica gel chromatography on a Biotage™ flash chromatography system using a gradient of 0-10% methanol in ethyl acetate. Compound 76 was obtained as a colorless gum (652 mg, 92%). 1H NMR (400 MHz, CDCl3) δ 1.44 (s, 9H), 2.22-2.37 (m, 2H), 2.57 (t, J=6.4 Hz, 2H), 2.62 (tt, J=23.9, 7.0 Hz, 1H), 3.03-3.09 (m, 1H), 3.14-3.20 (m, 1H), 3.77-3.93 (m, 4H), 4.58-4.63 (m, 8H), 4.66-4.71 (m, 1H), 5.21-5.26 (m, 4H), 5.33-5.41 (m, 4H), 5.89-5.99 (m, 4H), 6.58-6.66 (m, 2H), 7.09 (d, J=8.2, 1H), 7.19-7.23 (m, 3H), 7.27-7.30 (m, 2H).

Workup

  1. stirringstirring
  2. waitwas pursued for 2.5 h at 0° C
  3. washwashed with H2O, 1N HCl solution, saturated NaHCO3 solution and saturated NaCl solution
  4. dry with materialThe organic layer was dried over MgSO4
  5. filtrationfiltered
  6. concentrationconcentrated to dryness
  7. customThe crude product was purified by silica gel chromatography on a Biotage™ flash chromatography system

Sources & evidence

ORD record
ord-ff1ade8889734ef28619030a99ed52be
Source dataset
uspto-grants-2013_09
Patent number
US08524691B2

Linked notes

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