[CH2:1]([N:3]1[C:7]2=[N:8][CH:9]=[C:10]([C:15]([OH:17])=O)[C:11]([C:12](O)=[O:13])=[C:6]2[CH:5]=[N:4]1)[CH3:2].CCN=C=NCCCN(C)C.Cl.C1C=CC2N(O)[N:37]=[N:36]C=2C=1.C(N(CC)CC)C.NN>C1COCC1.O>[CH2:1]([N:3]1[C:7]2[N:8]=[CH:9][C:10]3[C:15](=[O:17])[NH:36][NH:37][C:12](=[O:13])[C:11]=3[C:6]=2[CH:5]=[N:4]1)[CH3:2]An alternative method could be used by starting with 1-ethyl-1H-pyrazolo[3,4-b]pyridine-4,5-dicarboxylic acid. The diacid was dissolved in anhydrous THF with EDAC.HCl (1.2 eq), HOBT (1.2 eq), and triethylamine (5.0 eq). To this solution was added anhydrous hydrazine (2.0 eq). The resulting solution was stirred at rt for 5 h and then diluted with water. The precipitated solid was collected by filtration to afford the title compound as a white solid.
Loading…