Staurosporine → Staurosporine
Reaction equation
CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4.Cc1cc(C=O)no1>CC(=O)O.CC(=O)O[BH-](OC(C)=O)OC(C)=O.CC(C)[O-].CC(C)[O-].CC(C)[O-].CC(C)[O-].CN1CCCC1=O.[Na+].[Ti+4]>CO[C@@H]1[C@H](N(C)Cc2cc(C)on2)C[C@H]2O[C@]1(C)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4Participants
9
Reactant 2
Product 3
Reagents, catalysts & solvents 4
Conditions
- Temperature
- 22 °C
Workup
- additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
- additionThe reactions were quenched with 1 uL saturated aqueous NaHCO3.
- additionThe reactions were quenched with 1 uL water.
- additionThe reactions were diluted to approximately 10 mM.
- aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
- additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
Sources & evidence
- ORD record
- ord-8344bf4420954828aaf53460728e9586
- Source dataset
- Cernak miniaturized reductive amination
- Original link
- View source
Reaction notes
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