Trifluoroacetic acid → (6R)-3-[(2-amino-[1,3]thiazolo[4,5-c]pyridin-5-ium-5-yl)methyl]-7-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(1-carboxycyclobutyl)oxyiminoacetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate

A mixture of benzhydryl 7-[(Z)-2-(1-t-butoxycarbonylcyclobut-1-oxyimino)-2-(2-tritylaminothiazol-4-yl)-acetamido]-3-iodomethyl-3-cephem-4-carboxylate [VIIe'] (643 mg, 0.6 mmole) and 2-aminothiazolo[4,5-c]pyridine (100 mg, 0.67 mmole) in 3 ml of dry DMSO was stirred at room temperature for an hour. The reaction mixture was diluted with ether (50 ml) to separate a precipitate, which was triturated with ether (50 ml). The resulting precipitate was dissolved in 30 ml of methylene chloride. After filtration, the filtrate was evaporated and the residue was triturated with isopropyl ether to give 622 mg of the crude blocked quaternary salt as a brown powder. A mixture of the crude solid (450 mg, 0.37 mmole), anisole (0.5 ml), trifluoroacetic acid (5 ml), and water (0.3 ml) was stirred at room temperature for 1.25 hours and concentrated under reduced pressure. The residue was triturated with isopropyl ether (70 ml), and the precipitate was isolated by filtration to give 330 mg of crude final product as a yellow powder, which was dissolved in a mixture of 85% formic acid (1 ml) and concentrated hydrochloric acid (0.05 ml). The solution was allowed to stand at room temperature for 20 minutes and adsorbed on a column of HP-20 resin (40 ml). It was eluted with water (150 ml), 30% aqueous methanol (150 ml) and 50% aqueous methanol (300 ml). Fractions containig the desired product were combined, concentrated and lyophilized to give 167 mg of powder, which was found to be a mixture of the Δ2 and Δ3 isomers by NMR and HPLC. It was further purified by column chromatography using the packing of the PrepPAK-500/C18 (Waters) (20 ml) and the eluate with water (400 ml) was collected in 10 ml fractions, which were monitored by HPLC. The desired fractions were combined, concentrated and lyophilized to afford 58 mg (20%) of Ik as a pale yellow amorphous powder. Mp >150° C. (dec.). Estimated purity 80% (by HPLC).

1. customto separate a precipitate, which
2. customwas triturated with ether (50 ml)
3. dissolutionThe resulting precipitate was dissolved in 30 ml of methylene chloride
4. filtrationAfter filtration
5. customthe filtrate was evaporated
6. customthe residue was triturated with isopropyl ether
7. customto give 622 mg of the crude
8. stirringwas stirred at room temperature for 1.25 hours
9. concentrationconcentrated under reduced pressure
10. customThe residue was triturated with isopropyl ether (70 ml)
11. customthe precipitate was isolated by filtration
12. customto give 330 mg of crude final product as a yellow powder, which
13. washIt was eluted with water (150 ml), 30% aqueous methanol (150 ml) and 50% aqueous methanol (300 ml)
14. concentrationconcentrated

ORD record

ord-f2809593d6c04da8b22e1ba11a9daae1

Source dataset

uspto-grants-1985_02

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US04500526