Uracil → D-xylonic acid

HRID 630589Notes

Reaction equation

HRID 630589 reaction equation
N1C=CC(=O)NC1=[O:3].O=C[C@@H:11]([C@H:13]([C@@H:15]([C@@H:17]([CH2:19][OH:20])[OH:18])[OH:16])[OH:14])[OH:12].O=C[C@@H]([C@H]([C@@H](CO)O)O)O>>[O:3]=[C:11]([OH:12])[C@@H:13]([C@H:15]([C@@H:17]([CH2:19][OH:20])[OH:18])[OH:16])[OH:14]

Participants

4

Reactant 3

HCID 1174UracilHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 644160aldehydo-D-Xylose

Product 1

HCID 6602431D-xylonic acid

Procedure

The resulting strains H3679 and H3680 were cultured on SC-medium lacking uracil for plasmid selection, with 10 g I−1 glucose and 20 g I−1 xylose as carbon sources. The strains were cultured in 250 ml Erlenmeyer flasks in 50 ml medium with 250 rpm agitation at 30° C. Strain H3679 expressing the gene CC_1225 produced 3.3 g xylonic acid I−1 extracellularly in 75 h (FIG. 14). Strain H3680 (control strain) produced no xylonic acid when grown under the same conditions.

Workup

  1. customwere cultured in 250 ml Erlenmeyer flasks in 50 ml medium with 250 rpm agitation at 30° C

Sources & evidence

ORD record
ord-709420b040f44d23ac85a109df124b37
Source dataset
uspto-grants-2016_04
Patent number
US09315834B2

Reaction notes

Loading…