N,O-Dimethylhydroxylamine → 5-bromo-N,2-dimethoxy-N-methylnicotinamide
Reaction equation
CNOC.COc1ncc(Br)cc1C(=O)O.CCN=C=NCCCN(C)C.Cl.C1=CC=C2C(=C1)N=NN2O.CC1=NC(=CC=C1)C.CN(C)C=O>>COc1ncc(Br)cc1C(=O)N(C)OCParticipants
7
Reactant 2
Product 1
Reagents, catalysts & solvents 4
Conditions
- Temperature
- 25 °C
Workup
- addition150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200.
- addition150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200.
- aliquot10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate.
- addition380 uL MeCN solvent were added to the transfer plate
- aliquot20 uL taken from transfer plate to filter plate 1 for LCMS analysis
- addition180 uL MeCN added to filter plate 1 for LCMS analysis
- aliquot10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis
- addition190 uL MeCN added to filter plate 2 for TOF-DESI analysis
- customthe 96-well filter plates were centrifuged for 2.5 min at 3000 RPM
Sources & evidence
- ORD record
- ord-91237f9e4f744c1ea524f32f174ba686
- Source dataset
- AIChemEco amide coupling conditions 47k dataset
Reaction notes
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