L-Valine → L-Lysine

HRID 1440302

反応式

HRID 1440302 の反応式
C[C@H]1O[C@H]2O[C@H:10]3[C@H:15](O[C@@]2(O)C(=O)C1)[C@@H:14]([NH:17]C)[C@@H:13]([OH:19])[C@@H:12]([NH:20]C)[C@@H:11]3O.[OH:24]C1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O.OP([O-])(O)=O.[K+].OP([O-])([O-])=O.[K+].[K+].[O-]S([O-])(=O)=O.[Mg+2].OC(CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=O.C([O-])([O-])=O.[Ca+2].N[C@H](C(O)=O)CCO.N[C@H](C(O)=O)[C@@H](C)O.N[C@H](C(O)=O)[C@H](CC)C.N[C@H](C(O)=O)C(C)C>[O-]S([O-])(=O)=O.[Mn+2]>[NH2:17][C@H:14]([C:13]([OH:24])=[O:19])[CH2:15][CH2:10][CH2:11][CH2:12][NH2:20]

参加物

13

反応物 11

HCID 6287L-ValineHCID 6288L-ThreonineHCID 6306L-IsoleucineHCID 10112Calcium CarbonateHCID 12647L-HomoserineHCID 15541SpectinomycinHCID 24083Sulfuric acid magnesium salt (1:1)HCID 24450Potassium Phosphate, DibasicHCID 171548BiotinHCID 440995(+)-LactoseHCID 516951Monobasic potassium phosphate

生成物 1

HCID 5962L-Lysine

試薬・触媒・溶媒 1

HCID 24580Manganese Sulfate触媒

実験手順

Each strain was cultured in NB medium at 30° C. for 16 hours. For the culture of the transformants, spectinomycin was added to NB medium at a concentration of 100 μg/ml. Then, 0.5 ml of the seed culture was inoculated into 5 ml of a production medium (pH 7.2) comprising 100 g/l lactose, 30 g/l (NH4)2SO4, 0.5 g/l KH2PO4, 0.5 g/l K2HPO4, 1 g/l MgSO4 ·7H2O, 10 mg/l FeSO4 ·7H2O, 10 mg/l MnSO4 ·7H2O, 100 μg/l biotin and 30 g/l CaCO3 in a test tube. Homoserine (100 μg/ml) was further added to the production medium for RH6 strain and its transformant, and 100 μg/ml each of threonine, isoleucine and valine were added to the production medium for ATCC 21086 strain and its transformant. Shaking culture was carried out at 30° C. for 72 hours. After the culturing was finished, the amount of L-lysine formed was colorimetrically determined by the acidic copper ninhydrin method [Chinard, J. Biol. Chem., 199, 91 (1952)]. The results are shown in Table 7.

後処理

  1. additionwere added to the production medium for ATCC 21086 strain and its transformant
  2. waitwas carried out at 30° C. for 72 hours

出典と根拠

ORDレコード
ord-2f452d3bab23462a84f5f99d56ff15b2
出典データセット
uspto-grants-1996_03
特許番号
US05498532

関連ノート

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