Fructose 6-phosphate → D-(-)-Fructose

HRID 1755210

反応式

HRID 1755210 の反応式
P([O:5][CH2:6][C@@H:7]([OH:16])[C@@H:8]([OH:15])[C@H:9]([OH:14])[C:10](=[O:13])[CH2:11][OH:12])(O)(O)=O.C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1.P(OC[C@@H](O)[C@@H](O)C=O)(O)(O)=O>C(O)C(N)(CO)CO.Cl>[OH:12][CH2:11][C:10]([C@H:9]([C@@H:8]([C@@H:7]([CH2:6][OH:5])[OH:16])[OH:15])[OH:14])=[O:13]

参加物

5

反応物 3

HCID 69507Fructose 6-phosphateHCID 122357Erythrose, 4-phosphateHCID 439153Nadh

生成物 1

HCID 5984D-(-)-Fructose

試薬・触媒・溶媒 1

HCID 93573Tromethamine hydrochloride溶媒2 回

実験手順

Cell disruption was performed in Tris/HCl buffer (100 mM, pH 7.8). The reaction for determination of transaldolase was performed in 50 mM Tris/HCl buffer (pH 7.8) additionally containing 1 mM fructose 6-phosphate, 0.5 mM NADH, 4 mM erythrose 4-phosphate, 6 U TPI/GDH and 50 μl of crude cell extract. TPI/GDH served as coupling enzymes to allow online monitoring at 340 nm.

後処理

  1. customThe reaction for determination of transaldolase
  2. extractionextract

出典と根拠

ORDレコード
ord-05dd12aaed8c491d922b32edbd1a7e07
出典データセット
uspto-grants-2015_10
特許番号
US09169502B2

関連ノート

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