Phenylmethylsulfonyl Fluoride → Coenzyme A

HRID 1871866

反応式

HRID 1871866 の反応式
CC1C=C2N=C3C(=NC(NC3=O)=O)N(C[C@H](O)[C@H](O)[C@H](O)CO)C2=CC=1C.N([S:48][CH2:49][CH2:50][NH:51][C:52](=[O:95])[CH2:53][CH2:54][NH:55][C:56](=[O:94])[C@H:57]([OH:93])[C:58]([CH3:92])([CH3:91])[CH2:59][O:60][P:61]([OH:90])(=[O:89])[O:62][P:63]([OH:88])(=[O:87])[O:64][CH2:65][C@H:66]1[O:70][C@@H:69]([N:71]2[C:80]3[N:79]=[CH:78][N:77]=[C:75]([NH2:76])[C:74]=3[N:73]=[CH:72]2)[C@H:68]([OH:81])[C@@H:67]1[O:82][P:83]([OH:86])([OH:85])=[O:84])[C@@H](CCC(N[C@H](C(NCC(O)=O)=O)CS)=O)C(O)=O.CC(C(O)C(NCCC(NCCS)=O)=O)(COP(OP(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(O)=O)(O)=O)C.[N+](C1C=CC(SSC2C=CC([N+]([O-])=O)=C(C=2)C(O)=O)=CC=1C(O)=O)([O-])=O.C1C=C2C(C(O)=O)=CC(C3C=C(C(O)=O)C4C(=CC=CC=4)N=3)=NC2=CC=1.C1(CS(F)(=O)=O)C=CC=CC=1.CC([C@@H](O)C(NCCC(NCCS)=O)=O)(COP(OP(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(O)=O)(O)=O)C>C(O)C(N)(CO)CO.Cl.O.O.O.O.O.S([O-])([O-])(=O)=O.[Cu+2].[Cu]>[CH3:92][C:58]([C@@H:57]([OH:93])[C:56]([NH:55][CH2:54][CH2:53][C:52]([NH:51][CH2:50][CH2:49][SH:48])=[O:95])=[O:94])([CH2:59][O:60][P:61]([O:62][P:63]([O:64][CH2:65][C@H:66]1[O:70][C@@H:69]([N:71]2[C:80]3[N:79]=[CH:78][N:77]=[C:75]([NH2:76])[C:74]=3[N:73]=[CH:72]2)[C@H:68]([OH:81])[C@@H:67]1[O:82][P:83]([OH:86])([OH:85])=[O:84])([OH:88])=[O:87])([OH:90])=[O:89])[CH3:91]

参加物

11

反応物 7

HCID 4784Phenylmethylsulfonyl FluorideHCID 62545,5'-Dithiobis(2-nitrobenzoic Acid)HCID 71068Bicinchoninic acidHCID 439335[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [3-hydroxy-2,2-dimethyl-4-oxo-4-[[3-oxo-3-(2-sulfanylethylamino)propyl]amino]butyl] hydrogen phosphateHCID 444485Dephospho-coaHCID 493570RiboflavinHCID 86755643glutathionyl-coenzyme A

生成物 1

HCID 87642Coenzyme A

試薬・触媒・溶媒 3

HCID 23978Copper触媒HCID 24463Copper sulfate pentahydrate触媒HCID 93573Tromethamine hydrochloride溶媒

実験手順

Reduced nicotinamide adenine dinucleotide phosphate (NADPH), riboflavin, flavin adenine mononucleotide (FMN), flavin adenine dinucleotide (FAD), coenzyme A disulfide, glutathionyl-coenzyme A mixed disulfide, 3'-dephospho coenzyme A, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB), lysostaphin, bicinchoninic acid solution, 4% copper sulphate pentahydrate solution, 2',5'-adenosine diphosphate (ADP)-Sepharose®; and phenylmethylsulphonyl fluoride (PMSF) were from Sigma (Mississauga, ON). 3'-dephospho CoA was oxidized to the disulfide by incubation at room temperature overnight in Tris-HCl (20 mM), pH 9.0, containing copper (5 μM). 4,4'-phosphopantetheine was formed by incubation of CoA with nucleotide pyrophosphatase. The thiol was oxidized as described above, and the disulfide was purified by high performance liquid chromatography (HPLC). All other chemicals were of reagent grade or better and were used without further purification.

出典と根拠

ORDレコード
ord-20e171cb10bc4349a82014739271f945
出典データセット
uspto-grants-2000_08
特許番号
US06107068

関連ノート

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