5'-Atp → L-Tryptophan

HRID 1885129

反応式

HRID 1885129 の反応式
C(O)[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3]O.Cl.C(S)[C@@H](O)[C@H]([OH:15])CS.[Mg+2].[Cl-].[Cl-].C1N=[C:25](N)[C:24]2N=C[N:30]([C@@H:31]3O[C@H:34]([CH2:36]OP(OP(O[32P](O)(O)=O)(O)=O)(O)=O)[C@@H:33](O)[C@H:32]3O)[C:23]=2N=1.P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>>[NH2:7][C@H:2]([C:5]([OH:15])=[O:6])[CH2:3][C:32]1[C:33]2[C:23](=[CH:24][CH:25]=[CH:36][CH:34]=2)[NH:30][CH:31]=1

参加物

6

反応物 5

HCID 59575'-Atp2 回HCID 93573Tromethamine hydrochloride2 回HCID 446094D-1,4-Dithiothreitol2 回HCID 644208(gamma-32P)-ATPHCID 5360315Magnesium Chloride2 回

生成物 1

HCID 6305L-Tryptophan

実験手順

The ten oligodeoxynucleotides shown in FIG. 3A were synthesized by the phosphotriester method and purified. 500 pmole of each oligodeoxynucleotide except 1 and 10 were phosphorylated individually in 20 μl containing 60 mM Tris-HCl, pH 8, 15 mM DTT, 10 mM MgCl2, 20 μCi of [γ-32P]-ATP and 20 units of polynucleotide kinase (P/L Biochemicals) for 30 min. at 37° C. This was followed by the addition of 10 μl containing 60 mM Tris-HCl, pH 8, 15 mM DTT, 10 mM MgCl2, 1.5 mM ATP and 20 additional units of polynucleotide kinase followed by another 30 min incubation at 37° C. Following incubation the samples were incubated at 100° C. for 5 min. 500 pmole of oligodeoxynucleotides 1 and 10 were diluted to 30 μl in the above buffer without ATP.

後処理

  1. custompurified
  2. additionThis was followed by the addition of 10 μl
  3. customat 37° C
  4. waitwere incubated at 100° C. for 5 min

出典と根拠

ORDレコード
ord-46dc5864740541618f00774b7bc195a2
出典データセット
uspto-grants-1992_09
特許番号
US05143829

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