L-(-)-Sorbose → Sorbosone, L-

HRID 2073158

反応式

HRID 2073158 の反応式
[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4].P([O-])([O-])([O-])=O.[K+].[K+].[K+].C[N+]1C2C(=CC=CC=2)N=C2C=1C=CC=C2.COS([O-])(=O)=O>O>[CH2:11]([OH:12])[C@H:9]([OH:10])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C:3]([CH:2]=[O:1])=[O:4]

参加物

5

反応物 3

HCID 6904L-(-)-Sorbose2 回HCID 9285Phenazine MethosulfateHCID 62657Potassium Phosphate, Tribasic

生成物 1

HCID 10921067Sorbosone, L-

試薬・触媒・溶媒 1

HCID 962Water溶媒

実験手順

The reaction mixture containing 100 ml of cell free extract of UV-10, FERM BP-1267 (total enzyme activity, 123 units), as prepared by the manner as described in steps (1) to (2) of Example 1, 50 ml of 0.5M potassium phosphate buffer (PH 6.0), 50 ml of 1M L-sorbose solution, 10 ml of 0.2M phenazine methosulfate solution and 290 ml of water was incubated at 30° C. with gentle shaking. As a result, L-sorbosone was formed with the rate of 142 mg/hr.

後処理

  1. additionThe reaction mixture containing 100 ml of cell
  2. extractionfree extract of UV-10, FERM BP-1267 (total enzyme activity, 123 units)
  3. customas prepared by the manner
  4. customwas incubated at 30° C. with gentle shaking

出典と根拠

ORDレコード
ord-09d3d758083f48dcb63646c3023180fe
出典データセット
uspto-grants-1990_04
特許番号
US04916069

関連ノート

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