L-(-)-Sorbose → Sorbosone, L-

HRID 2073159

反応式

HRID 2073159 の反応式
[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4].P([O-])([O-])([O-])=O.[K+].[K+].[K+]>C[N+]1C2C(=CC=CC=2)N=C2C=1C=CC=C2.COS([O-])(=O)=O>[CH2:11]([OH:12])[C@H:9]([OH:10])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C:3]([CH:2]=[O:1])=[O:4]

参加物

4

反応物 2

HCID 6904L-(-)-Sorbose3 回HCID 62657Potassium Phosphate, Tribasic

生成物 1

HCID 10921067Sorbosone, L-

試薬・触媒・溶媒 1

HCID 9285Phenazine Methosulfate溶媒

実験手順

The reaction mixture containing 400 ml of purified membrane-bound L-sorbose dehydrogenase (total activity, 120 units), as prepared by the manner as described in steps (1) to (5) of Example 1, 50 ml of 0.5M potassium phosphate buffer (pH 6.0). 50 ml of 1M L-sorbose solution and 10 ml of 0.2M phenazine methosulfate solution was incubated at 30° C. with gentle shaking. As a result, L-sorbosone was formed with the rate of 224 mg/hr.

後処理

  1. customas prepared by the manner
  2. customwas incubated at 30° C. with gentle shaking

出典と根拠

ORDレコード
ord-9f0bf67697f44edd8787cd0b3876adcd
出典データセット
uspto-grants-1990_04
特許番号
US04916069

関連ノート

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