Uridine Diphosphate → Uridine 5'-triphosphate

反応式

[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[CH:23]=[CH:22][C:20](=[O:21])[NH:19][C:17]2=[O:18])[C@H:13]([OH:24])[C@@H:12]1[OH:25])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:3])[OH:2].[Cl-].[Mg+2].[Cl-].C(N(CCCC)CCCC)CCC>CN(C)C=O>[CH:22]1[C:20](=[O:21])[NH:19][C:17](=[O:18])[N:16]([C@@H:14]2[O:15][C@H:11]([CH2:10][O:9][P:1]([O:4][P:5]([O:8][P:1]([OH:4])([OH:3])=[O:2])([OH:7])=[O:6])([OH:2])=[O:3])[C@@H:12]([OH:25])[C@H:13]2[OH:24])[CH:23]=1

参加物

実験手順

A 0.154 M dimethylformamide solution of uridine 5′-diphosphate (UDP) tributylamine salt (584 μL, 90.0 μmol) and a 0.45 M dimethylformamide solution of anhydrous magnesium chloride (200 μL, 90.0 μmol) were added to a 0.15 M dimethylformamide solution of cUTP tributylamine salt (500 μL, 75.0 μmol), and the mixture was stirred at room temperature for 72 hours. The resultant reaction mixture was analyzed through HPLC (262 nm). As a result, Up5U (i.e., target product) was found to be produced from UTP at a synthesis yield of 50.5%.