Uridine Diphosphate → Uridine 5'-triphosphate

HRID 217098

反応式

HRID 217098 の反応式
[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[CH:23]=[CH:22][C:20](=[O:21])[NH:19][C:17]2=[O:18])[C@H:13]([OH:24])[C@@H:12]1[OH:25])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:3])[OH:2].[Cl-].[Mg+2].[Cl-].C(N(CCCC)CCCC)CCC>CN(C)C=O>[CH:22]1[C:20](=[O:21])[NH:19][C:17](=[O:18])[N:16]([C@@H:14]2[O:15][C@H:11]([CH2:10][O:9][P:1]([O:4][P:5]([O:8][P:1]([OH:4])([OH:3])=[O:2])([OH:7])=[O:6])([OH:2])=[O:3])[C@@H:12]([OH:25])[C@H:13]2[OH:24])[CH:23]=1

参加物

5

反応物 3

HCID 6031Uridine DiphosphateHCID 7622TributylamineHCID 5360315Magnesium Chloride

生成物 1

HCID 6133Uridine 5'-triphosphate収率 50.5%

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒3 回

実験手順

A 0.154 M dimethylformamide solution of uridine 5′-diphosphate (UDP) tributylamine salt (584 μL, 90.0 μmol) and a 0.45 M dimethylformamide solution of anhydrous magnesium chloride (200 μL, 90.0 μmol) were added to a 0.15 M dimethylformamide solution of cUTP tributylamine salt (500 μL, 75.0 μmol), and the mixture was stirred at room temperature for 72 hours. The resultant reaction mixture was analyzed through HPLC (262 nm). As a result, Up5U (i.e., target product) was found to be produced from UTP at a synthesis yield of 50.5%.

後処理

  1. customThe resultant reaction mixture

出典と根拠

ORDレコード
ord-b779d8eddbca477eb784101004ed9b6e
出典データセット
uspto-grants-2012_06
特許番号
US08193338B2

関連ノート

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