Formamide → Fluorescein 5-isothiocyanate

HRID 266686

反応式

HRID 266686 の反応式
[C:1]([O-:13])(=O)[CH2:2][C:3]([CH2:8][C:9]([O-:11])=O)(C([O-])=O)[OH:4].[Na+].[Na+].[Na+].[CH:17]([NH2:19])=O.CCC(C[O:26][C:27](C(N(CC[NH+](C)C)C)=O)([C:34]1[CH:39]=[CH:38][CH:37]=[CH:36][CH:35]=1)C1C=CC=CC=1)CC.[Cl-].[N-]=C=S.NC(N)=N.P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+].C(N([CH2:81][C:82](O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O.C(S)[C@@H](O)[C@H](O)C[SH:89].C(O)[C@H]1O[C@H](O[C@:101]2([CH2:110]O)[O:105][C@H:104]([CH2:106]O)[C@@H:103](O)[C@@H:102]2O)[C@H](O)[C@@H](O)[C@@H]1O>O.CN(C)C=O>[CH:37]1[C:36]([N:19]=[C:17]=[S:89])=[CH:35][C:34]2[C:27]([O:11][C:9]3([C:8]4[CH:81]=[CH:82][C:1]([OH:13])=[CH:2][C:3]=4[O:4][C:110]4[CH:106]=[C:104]([OH:105])[CH:103]=[CH:102][C:101]3=4)[C:39]=2[CH:38]=1)=[O:26]

参加物

12

反応物 9

HCID 713FormamideHCID 5988Sucrose2 回HCID 6049Ethylenediaminetetraacetic AcidHCID 6224Trisodium citrateHCID 19153X 1002 回HCID 24243Trisodium PhosphateHCID 446094D-1,4-DithiothreitolHCID 178435168HCID 178435168、化合物HCID 178513176HCID 178513176、化合物

生成物 1

HCID 18730Fluorescein 5-isothiocyanate

試薬・触媒・溶媒 2

HCID 962Water溶媒2 回HCID 6228Dimethylformamide溶媒

実験手順

5× SSC (0.75M NACl, 0.075M sodium citrate); 30% Formamide (v/v); 3% Triton X-100 (v/v) (Triton X-100 is an alcohol derivative of polyoxyethylene ether, see Aldrich Chemical Co. catalogue for 1990-91); 0.4M Guanidine isothiocyanate; 0.16M sodium phosphate buffer (pH 6); 15× Ficoll/PVP (polysucrose 400,000 mol wt/polyvinyl pyrrolidone); 1 mg/ml Sheared Salmon Sperm DNA; 10 mM EDTA; 25 mM DTT; 5% PEG 4000. In the foregoing, 500× Ficoll/PVP is 5 g of Ficoll type 400 (polysucrose 400,00 mol wt) plus 5 g of PVP (polyvinylpyrrolidone) dissolved in water to a total volume of 100 ml; 15× Ficoll/PVP indicates that 500× Ficoll/PVP has been diluted by a factor of 15/500 with water. Ficoll (Pharmacia) is a nonionic synthetic polymer of sucrose. A stock solution of 0.1 mg/ml FITC in dimethylformamide was prepared. It was added to the HC in a quantity to produce a final concentration of FITC in the HC of 2 μg/ml.

後処理

  1. additionIt was added to the HC in a quantity

出典と根拠

ORDレコード
ord-311d916eed684cbeb6f710ec2a2e6c03
出典データセット
uspto-grants-1997_07
特許番号
US05652093

関連ノート

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