反応式
N[C@@H](CCC(N[C@H](C(NCC(O)=O)=O)CS)=O)C(O)=O.C(N(CC([O-])=O)CC(O)=O)CN(CC([O-])=O)CC(O)=O.[Na+].[Na+].[CH:43]1[CH:48]=[N+:47]([C@@H:49]2[O:53][C@H:52]([CH2:54][O:55][P:56]([O:59][P:60]([O:63][CH2:64][C@H:65]3[O:69][C@@H:68]([N:70]4[C:74]5[N:75]=[CH:76][N:77]=[C:78]([NH2:79])[C:73]=5[N:72]=[CH:71]4)[C@H:67]([O:80][P:81]([OH:84])([OH:83])=[O:82])[C@@H:66]3[OH:85])([OH:62])=[O:61])([OH:58])=[O:57])[C@@H:51]([OH:86])[C@H:50]2[OH:87])[CH:46]=[C:45]([C:88]([NH2:90])=[O:89])[CH:44]=1.[Cl-].[Mg+2].[Cl-].C(OP(O)(O)=O)[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O.P(OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)(O)(O)=O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH:76]1[N:77]=[C:78]([NH2:79])[C:73]2[N:72]=[CH:71][N:70]([C@@H:68]3[O:69][C@H:65]([CH2:64][O:63][P:60]([O:59][P:56]([O:55][CH2:54][C@H:52]4[O:53][C@@H:49]([N:47]5[CH:46]=[C:45]([C:88]([NH2:90])=[O:89])[CH2:44][CH:43]=[CH:48]5)[C@H:50]([OH:87])[C@@H:51]4[OH:86])([OH:58])=[O:57])([OH:62])=[O:61])[C@@H:66]([OH:85])[C@H:67]3[O:80][P:81]([OH:84])([OH:83])=[O:82])[C:74]=2[N:75]=1参加物
実験手順
The enzymic activity is determined according to the following procedure: 1.72 g of glutathione, and 34.2 mg of Na2EDTA are dissolved in a 114 mM potassium phosphate buffer solution (pH 7.0) to prepare 100 ml of a solution. A mixture of 700 μl of the solution, 700 μl of a thermally dissolved 5.72% starch solution, 100 μl of a 20 mM NADP+ solution, 100 μl of a 26 mM magnesium chloride solution, 100 μl of a 1.34 mM glucose 1,6-diphosphate solution, 100 μl of a 31 unit/ml phosphoglucomutase solution, 100 μl of a 35 unit/ml glucose 6-phosphate dehydrogenase solution, and 100 μl of a sample enzyme solution is incubated at 30° C. and the quantity of produced NADPH is monitored consecutively by means of absorption spectrophotometry at 340 nm. One unit refers to the amount of enzyme which is capable of producing 1 μ mol of NADPH for 1 min. under the conditions.
後処理
- customis incubated at 30° C.
- customthe quantity of produced NADPH is monitored consecutively by means of absorption spectrophotometry at 340 nm
出典と根拠
- ORDレコード
- ord-a6765ea1d7194d5cada5702e2f668ad5
- 出典データセット
- uspto-grants-1996_10
- 特許番号
- US05565341
関連ノート
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