Phosphate → aldehydo-D-Xylose

HRID 1154628

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HRID 1154628 반응식
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([CH2:9][OH:10])[OH:8])[OH:6])[OH:4].[Mg+2].[Cl-].[Cl-].P([O-])([O-])([O-])=O.[O:19]=[CH:20][C@@H:21]([C@H:23]([C@@H:25]([C@@H:27](CO)[OH:28])[OH:26])[OH:24])[OH:22]>O>[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([CH2:9][OH:10])[OH:8])[OH:6])[OH:4].[OH:19][CH2:20][C:21]([C@H:23]([C@@H:25]([CH2:27][OH:28])[OH:26])[OH:24])=[O:22]

참가 물질

7

반응물 4

HCID 1061PhosphateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 644160aldehydo-D-XyloseHCID 5360315Magnesium Chloride

생성물 2

HCID 644160aldehydo-D-XyloseHCID 5289590D-Xylulose

시약·촉매·용매 1

HCID 962Water용매

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온도
60 °C

실험 절차

Xylose (100 gm) was dissolved in distilled water to provide a concentration of 60% w/v. The solution was placed in a reaction vessel to which was added CoCl2 and MgCl2 to provide a concentration of Co++ =10-4M and Mg++ =10-3M. The pH of the solution was adjusted to 7.5 by the addition of a phosphate buffer. To the resulting solution was then added 5 gm of glucose isomerase extracted from Streptomyces and the temperature was maintained at 60° C. overnight (18 hours). The solution was then added to a fermentation medium (DIFCO 8.5 gm/l yeast extract, NH4Cl1.3 gm/l, MgSO4 ·7 H2O·11 gm/l, and CaCl2 .06 gm/l) to yield an initial fermentation substrate concentration of 78 gm/l xylose and 20 gm/l xylulose. The resulting pH was 5.5.

후처리

  1. customto provide a concentration of 60% w/v
  2. customThe solution was placed in a reaction vessel to which
  3. customto provide a concentration of Co++ =10-4M and Mg++ =10-3M
  4. extractionextracted from Streptomyces
  5. additionThe solution was then added to a fermentation medium (DIFCO 8.5 gm/l yeast extract, NH4Cl1.3 gm/l, MgSO4 ·7 H2O·11 gm/l, and CaCl2 .06 gm/l)

출처 및 근거

ORD 기록
ord-e6eea9964d9a4e8896b2697077a4e883
출처 데이터셋
uspto-grants-1984_12
특허 번호
US04490468

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