Phenylmethylsulfonyl Fluoride → Coenzyme A

HRID 1871866

반응식

HRID 1871866 반응식
CC1C=C2N=C3C(=NC(NC3=O)=O)N(C[C@H](O)[C@H](O)[C@H](O)CO)C2=CC=1C.N([S:48][CH2:49][CH2:50][NH:51][C:52](=[O:95])[CH2:53][CH2:54][NH:55][C:56](=[O:94])[C@H:57]([OH:93])[C:58]([CH3:92])([CH3:91])[CH2:59][O:60][P:61]([OH:90])(=[O:89])[O:62][P:63]([OH:88])(=[O:87])[O:64][CH2:65][C@H:66]1[O:70][C@@H:69]([N:71]2[C:80]3[N:79]=[CH:78][N:77]=[C:75]([NH2:76])[C:74]=3[N:73]=[CH:72]2)[C@H:68]([OH:81])[C@@H:67]1[O:82][P:83]([OH:86])([OH:85])=[O:84])[C@@H](CCC(N[C@H](C(NCC(O)=O)=O)CS)=O)C(O)=O.CC(C(O)C(NCCC(NCCS)=O)=O)(COP(OP(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(O)=O)(O)=O)C.[N+](C1C=CC(SSC2C=CC([N+]([O-])=O)=C(C=2)C(O)=O)=CC=1C(O)=O)([O-])=O.C1C=C2C(C(O)=O)=CC(C3C=C(C(O)=O)C4C(=CC=CC=4)N=3)=NC2=CC=1.C1(CS(F)(=O)=O)C=CC=CC=1.CC([C@@H](O)C(NCCC(NCCS)=O)=O)(COP(OP(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(O)=O)(O)=O)C>C(O)C(N)(CO)CO.Cl.O.O.O.O.O.S([O-])([O-])(=O)=O.[Cu+2].[Cu]>[CH3:92][C:58]([C@@H:57]([OH:93])[C:56]([NH:55][CH2:54][CH2:53][C:52]([NH:51][CH2:50][CH2:49][SH:48])=[O:95])=[O:94])([CH2:59][O:60][P:61]([O:62][P:63]([O:64][CH2:65][C@H:66]1[O:70][C@@H:69]([N:71]2[C:80]3[N:79]=[CH:78][N:77]=[C:75]([NH2:76])[C:74]=3[N:73]=[CH:72]2)[C@H:68]([OH:81])[C@@H:67]1[O:82][P:83]([OH:86])([OH:85])=[O:84])([OH:88])=[O:87])([OH:90])=[O:89])[CH3:91]

참가 물질

11

반응물 7

HCID 4784Phenylmethylsulfonyl FluorideHCID 62545,5'-Dithiobis(2-nitrobenzoic Acid)HCID 71068Bicinchoninic acidHCID 439335[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [3-hydroxy-2,2-dimethyl-4-oxo-4-[[3-oxo-3-(2-sulfanylethylamino)propyl]amino]butyl] hydrogen phosphateHCID 444485Dephospho-coaHCID 493570RiboflavinHCID 86755643glutathionyl-coenzyme A

생성물 1

HCID 87642Coenzyme A

시약·촉매·용매 3

HCID 23978Copper촉매HCID 24463Copper sulfate pentahydrate촉매HCID 93573Tromethamine hydrochloride용매

실험 절차

Reduced nicotinamide adenine dinucleotide phosphate (NADPH), riboflavin, flavin adenine mononucleotide (FMN), flavin adenine dinucleotide (FAD), coenzyme A disulfide, glutathionyl-coenzyme A mixed disulfide, 3'-dephospho coenzyme A, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB), lysostaphin, bicinchoninic acid solution, 4% copper sulphate pentahydrate solution, 2',5'-adenosine diphosphate (ADP)-Sepharose®; and phenylmethylsulphonyl fluoride (PMSF) were from Sigma (Mississauga, ON). 3'-dephospho CoA was oxidized to the disulfide by incubation at room temperature overnight in Tris-HCl (20 mM), pH 9.0, containing copper (5 μM). 4,4'-phosphopantetheine was formed by incubation of CoA with nucleotide pyrophosphatase. The thiol was oxidized as described above, and the disulfide was purified by high performance liquid chromatography (HPLC). All other chemicals were of reagent grade or better and were used without further purification.

출처 및 근거

ORD 기록
ord-20e171cb10bc4349a82014739271f945
출처 데이터셋
uspto-grants-2000_08
특허 번호
US06107068

연결된 노트

불러오는 중…