Hydrogen Peroxide → (R)-5-((1-amino-1-oxobutan-2-yl)amino)-3-(pyrazolo[1,5-a]pyridin-3-ylamino)pyrazine-2-carboxamide

HRID 2200513노트

반응식

HRID 2200513 반응식
Cl[C:2]1[N:7]=[C:6]([NH:8][C@H:9]([CH2:13][CH3:14])[C:10]([NH2:12])=[O:11])[CH:5]=[N:4][C:3]=1[C:15]#[N:16].Cl.Cl.[N:19]1[N:23]2[CH:24]=[CH:25][CH:26]=[CH:27][C:22]2=[C:21]([NH2:28])[CH:20]=1.C(=O)([O-])[O-:30].[Cs+].[Cs+].C1C=CC(P(C2C(C3C(P(C4C=CC=CC=4)C4C=CC=CC=4)=CC=C4C=3C=CC=C4)=C3C(C=CC=C3)=CC=2)C2C=CC=CC=2)=CC=1.[OH-].[K+].OO>O1CCOCC1.CO.CS(C)=O.CC([O-])=O.CC([O-])=O.[Pd+2]>[NH2:12][C:10](=[O:11])[C@H:9]([NH:8][C:6]1[N:7]=[C:2]([NH:28][C:21]2[CH:20]=[N:19][N:23]3[CH:24]=[CH:25][CH:26]=[CH:27][C:22]=23)[C:3]([C:15]([NH2:16])=[O:30])=[N:4][CH:5]=1)[CH2:13][CH3:14]

참가 물질

11

반응물 6

HCID 784Hydrogen PeroxideHCID 10796Cesium carbonateHCID 14797Potassium HydroxideHCID 634876Binap, (A+-)-HCID 74889838Pyrazolo[1,5-a]pyridin-3-amine dihydrochlorideHCID 86685581(R)-2-(6-chloro-5-cyanopyrazin-2-ylamino)butanamide

생성물 1

HCID 86685629(R)-5-((1-amino-1-oxobutan-2-yl)amino)-3-(pyrazolo[1,5-a]pyridin-3-ylamino)pyrazine-2-carboxamide수율 9.4%

시약·촉매·용매 4

HCID 167845palladium(II) acetate촉매HCID 679Dimethyl Sulfoxide용매HCID 887Methanol용매HCID 312751,4-Dioxane용매

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온도
115 °C

실험 절차

The mixture of (R)-2-((6-chloro-5-cyanopyrazin-2-yl)amino)butanamide (65 mg, 0.27 mmol), pyrazolo[1,5-a]pyridin-3-amine dihydrochloride (56 mg, 0.27 mmol), powder cesium carbonate (360 mg, 1.08 mmol), BINAP (31 mg, 0.05 mmol), Pd(OAc)2 (11 mg, 0.05 mmol) in 15 mL dioxane was degassed with argon stream. It was stirred in argon atmosphere at 115° C. for 16 h. The mixture was cooled, diluted with 100 mL EtOAc, vigorously stirred, and filtered through celite. The filtrate was concentrated and subjected to silica flash column with 0-8% MeOH in DCM to isolate the coupling product. It was dissolved in 8 mL MeOH and 2 mL DMSO. To the solution were added KOH (100 mg) and then 1 mL of H2O2 (50%). The mixture was stirred at RT for 30 m, quenched with acetonitrile and then TFA, concentrated in vacuo and subjected to reverse phase preparative HPLC to isolate the title compound (9 mg). MS found for C16H18N8O2 as (M+H)+355.4. UV: λ=297 nm. 1H NMR: (CD3OD) δ 8.33 (2H, m), 7.46 (1H, d, J=9.2 Hz), 7.32 (1H, s), 7.09 (1H, dd, J=8.8; 6.8 Hz), 6.78 (1H, t, J=7.2 Hz), 4.18 (1H, m), 1.83 (1H, m), 1.68 (1H, m), 0.92 (3H, t, J=7.2 Hz) ppm.

후처리

  1. customwas degassed with argon stream
  2. temperatureThe mixture was cooled
  3. additiondiluted with 100 mL EtOAc
  4. stirringvigorously stirred
  5. filtrationfiltered through celite
  6. concentrationThe filtrate was concentrated
  7. customto isolate the coupling product
  8. stirringThe mixture was stirred at RT for 30 m
  9. customquenched with acetonitrile
  10. concentrationTFA, concentrated in vacuo

출처 및 근거

ORD 기록
ord-fce0d02f78fd400e92521132eb8ea1c9
출처 데이터셋
uspto-grants-2016_06
특허 번호
US09359308B2

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