Udp-Glucose → 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose

HRID 467265

반응식

HRID 467265 반응식
[CH2:1]1[C@H:5]([N:6]2[C:12](=[O:13])[NH:11][C:9](=[O:10])[CH:8]=[CH:7]2)[O:4][C@H:3]([CH2:14][OH:15])[C@H:2]1[N:16]=[N+:17]=[N-:18].[O-:19][P:20]([O:23][P:24]([O:27]P([O-])([O-])=O)([O-:26])=[O:25])(=[O:22])[O-:21].[CH2:32]([OH:47])[C@H:33]1[O:38][C@H:37](OP(O)(O)=O)[C@H:36]([OH:44])[C@@H:35]([OH:45])[C@@H:34]1[OH:46].[Mg+2].[Cl-].[Cl-].C(O)C(N)(CO)CO.Cl.C1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP(O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=1.Cl(O)(=O)(=O)=O>>[N:16]([C@@H:2]1[C@@H:3]([CH2:14][OH:15])[O:4][C@@H:5]([N:6]2[CH:7]=[CH:8][C:9](=[O:10])[NH:11][C:12]2=[O:13])[CH2:1]1)=[N+:17]=[N-:18].[P:24]([O:27][C@H:33]([CH2:32][OH:47])[C@@H:34]([OH:46])[C@H:35]([OH:45])[C@@H:36]([OH:44])[CH:37]=[O:38])([O:23][P:20]([OH:21])([OH:19])=[O:22])([OH:26])=[O:25]

참가 물질

7

반응물 6

HCID 8629Udp-GlucoseHCID 24247Perchloric AcidHCID 65533alpha-D-glucose 1-phosphateHCID 93573Tromethamine hydrochlorideHCID 5360315Magnesium ChlorideHCID 131717599AzddU triphosphate

생성물 1

HCID 1317175983'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose

실험 절차

To 150 milligrams of AzddU-triphosphate was added 400 milligrams of glucose-1-phosphate, 6 millimole of MgCl2, 0.70 millimole of Tris-HCl pH 7.6, bacterial UDPG-pyrophosphorylase and inorganic pyrophosphatase. After 4 hours at room temperature, the reaction was stopped with perchloric acid (7N). After 30 minutes in ice, samples were centrifuged and the supernatant was neutralized with base. After another 30 minutes in ice, samples were recentrifuged and AzddU-5'-diphosphoglucose was purified by column chromatography.

후처리

  1. waitAfter 30 minutes in ice
  2. waitAfter another 30 minutes in ice
  3. customAzddU-5'-diphosphoglucose was purified by column chromatography

출처 및 근거

ORD 기록
ord-3cfdeae288a2409fb130096d830f42b3
출처 데이터셋
uspto-grants-1992_10
특허 번호
US05159067

연결된 노트

불러오는 중…