Thiamine → L-Glutamic Acid

HRID 500295

반응식

HRID 500295 반응식
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].OP([O-])(O)=O.[K+].[O-]S([O-])(=O)=O.[Mg+2].CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.[NH2:50][C@H:51]([C:56]([OH:58])=[O:57])[C@H:52](CC)[CH3:53].[C:59]([O-:62])([O-:61])=O.[Ca+2]>>[NH2:50][C@H:51]([C:56]([OH:58])=[O:57])[CH2:52][CH2:53][C:59]([OH:62])=[O:61]

참가 물질

8

반응물 7

HCID 1130ThiamineHCID 6306L-IsoleucineHCID 10112Calcium Carbonate2회HCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2회HCID 516951Monobasic potassium phosphateHCID 6097028Ammonium Sulfate

생성물 1

HCID 33032L-Glutamic Acid

실험 절차

Both strains, VL334thrC+ and VL334thrC+-ΔcsrA, can be grown for 18-24 hours at 37° C. on L-agar plates. Then, one loop of the cells can be transferred into test tubes containing 2 ml of fermentation medium. The fermentation medium contains 60 g/l glucose, 25 g/l ammonium sulfate, 2 g/l KH2PO4, 1 g/l MgSO4, 0.1 mg/ml thiamine, 70 μg/ml L-isoleucine and 25 μl CaCO3 (pH 7.2). Glucose and CaCO3 should be sterilized separately. Cultivation can be carried out at 30° C. for 3 days with shaking. After the cultivation, the amount of L-glutamic acid produced can be determined by paper chromatography (liquid phase composition: butanol-acetic acid-water=4:1:1) with subsequent staining by ninhydrin (1% solution in acetone) and further elution of the compounds in 50% ethanol with 0.5% CdCl2.

후처리

  1. additioncontaining 2 ml of fermentation medium
  2. waitCultivation can be carried out at 30° C. for 3 days

출처 및 근거

ORD 기록
ord-21c5c8ecc23b4d66a81d17036ff47001
출처 데이터셋
uspto-grants-2010_08
특허 번호
US07771976B2

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