反应详情

Potassium carbonate (6.0 mg, 43 μmol, 1.6 eq.), 18-crown-6 (11.4 mg, 43 μmol, 1.5 eq.) and methyl iodide (6.1 mg, 53 μmol, 2.0 eq.) were added to a solution of benzyl{(7R)-6-cyano-7-(4-cyanophenyl)-5-methyl-4-[3-(trifluoromethyl)phenyl]-4,7-dihydro[1,2,4]triazolo[1,5-a]pyrimidin-2-yl}carbamate (15 mg, 27 μmol) in DMF (2 ml). The reaction mixture was stirred at RT for 12 h and then concentrated under reduced pressure and purified by preparative HPLC (Gromsil C18 column, 30×250 mm; mobile phase: acetonitrile-water-0.1% TFA). After lyophilization, the product was obtained as a solid (13.3 mg, 86% of theory).

1. concentrationconcentrated under reduced pressure
2. custompurified by preparative HPLC (Gromsil C18 column, 30×250 mm; mobile phase: acetonitrile-water-0.1% TFA)
3. customAfter lyophilization, the product was obtained as a solid (13.3 mg, 86% of theory)