Anthranilic Acid → L-Tryptophan

HRID 1087003Notes

Reaction equation

HRID 1087003 reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.OP([O-])(O)=O.[K+].OC(C[CH2:23][CH2:24][CH2:25][C@H:26]1[C@@H:34]2[C@@H:29](N[C:31]([NH:33]2)=O)[CH2:28]S1)=O.CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.Cl.[NH2:54][C@H:55]([C:58]([OH:60])=[O:59])[CH2:56]O.C(O)(=O)C1C(=CC=CC=1)N.C([O-])([O-])=O.[Ca+2]>O>[NH2:54][C@H:55]([C:58]([OH:60])=[O:59])[CH2:56][C:28]1[C:29]2[C:34](=[CH:26][CH:25]=[CH:24][CH:23]=2)[NH:33][CH:31]=1

Participants

10

Reactant 8

HCID 227Anthranilic AcidHCID 313Hydrochloric AcidHCID 1130ThiamineHCID 5951L-SerineHCID 10112Calcium CarbonateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 516951Monobasic potassium phosphate

Product 1

HCID 6305L-Tryptophan

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

A culture medium containing 100 g of glucose, 40 g of (NH4)2SO4, 1 g of KH2PO4, 0.4 g of MgSO4.7H2O, 10 mg of FeSO4.7H2O, 10 mg of MnSO4.4H2O, 1 g of "Casamino acid", 300 microgram/l of biotin, 200 microgram/l of thiamine.HCl, 5 g of serine, 5 g of anthranilic acid and 50 g of CaCO3 in 1 l of water and having a pH value of 7.0 was used for the culture. Each the strains was inoculated into 3 ml of the above-mentioned culture medium placed in a test tube and then cultured at 30° C. for 70 hours with shaking. L-tryptophan in the supernatant of the culture liquid obtained by centrifugation after the culture was determined by a liquid chromatography.

Workup

  1. customplaced in a test tube

Sources & evidence

ORD record
ord-34336ab04fd54b17a0667f8bb4065b23
Source dataset
uspto-grants-1991_07
Patent number
US05034318

Reaction notes

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