Dimethyl Sulfoxide → L-Tyrosine

HRID 1091514Notes

Reaction equation

HRID 1091514 reaction equation
C(O)[C:2]([NH2:7])([CH2:5]O)[CH2:3][OH:4].Cl.[Mg+2].[Cl-].[Cl-].[CH2:13](S)[C@@H:14]([OH:19])[C@H:15](O)[CH2:16]S.P(O[CH2:34][C@H:35]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O.CS(C)=[O:54]>>[NH2:7][C@H:2]([C:3]([OH:4])=[O:54])[CH2:5][C:34]1[CH:16]=[CH:15][C:14]([OH:19])=[CH:13][CH:35]=1

Participants

6

Reactant 5

HCID 679Dimethyl Sulfoxide2×HCID 59575'-Atp2×HCID 93573Tromethamine hydrochlorideHCID 446094D-1,4-DithiothreitolHCID 5360315Magnesium Chloride

Product 1

HCID 6057L-Tyrosine

Procedure

All reagents were diluted with the Syk kinase assay buffer (50 mM Tris-HCl (pH 8.0), 10 mM MgCl2, 0.1 mM Na3VO4, 0.1% BSA, 1 mM DTT). First, a mixture (35 μl) including 3.2 μg of GST-Syk and 0.5 μg of AL was put in each well in 96-well plates. Then 5 μl of a test compound in the presence of 2.5% dimethyl sulfoxide (DMSO) was added to each well. To this mixture was added 300 μM ATP (10 μl) to initiate the kinase reaction. The final reaction mixture (50 pd) consists of 0.65 nM GST-Syk, 3 μM AL, 30 μM ATP, a test compound, 0.25% DMSO, and a Syk kinase assay buffer.

Workup

  1. customthe kinase reaction
  2. customThe final reaction mixture (50 pd)

Sources & evidence

ORD record
ord-1c82d7925f3845748788f208e2265d0d
Source dataset
uspto-grants-2012_03
Patent number
US08129386B2

Reaction notes

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