反应详情

500 mg of compound 46 and 145 mg of □-alaninamide hydrochloride are introduced into 10 ml of dichloromethane, and 0.8 ml of N,N-diisopropylethylamine, 215 mg of 1-hydroxybenzotriazole and 306 mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride are added. The solution is stirred for 12 h. The solution is concentrated and the crude product is purified by chromatography on silica gel (dichloromethane/methanol/glacial acetic acid 100:0:5→100:10:5). 440 mg of the desired compound 47 are obtained. C20H25N5O3 (383.5); MS (ESI+): 384.2 (M+H)+, tR=3.58 min (Gradient 3).

1. additionare added
2. concentrationThe solution is concentrated
3. customthe crude product is purified by chromatography on silica gel (dichloromethane/methanol/glacial acetic acid 100:0:5→100:10:5)