反应详情

The resin is suspended in 70 ml of activator solution (5% N-methyl-morpholine in DMF). Thereafter, added to the suspension are 8.2 g (14 mmoles) of bis-Fmoc-lysine, 7.28 g of coupling agent, benzotriazol-1-yl-oxy-tris-pyrro-lidinophosphonium hexafluorophosphate (PyBOP), and 2.14 g of catalyst, 1-hydroxybenzotriazole (HOBT). The reaction medium is mixed for 90 minutes and the resultant product is (Fmoc)2 -Lys-Ala-resin, i.e., (Fmoc)2 -K-A-resin. The product of the coupling reaction is washed 3 times with DCM, 1 time with methanol and 5 times with DMF and then subjected to deprotection using a mixture of 30% piperidine, 35% toluene and 35% DMF. The product obtained from the deprotection is Lys-Ala-resin (K-A-resin). This product is washed 5 times with DMF, 1 time with methanol and 3 times with DCM.

1. additionThereafter, added to the suspension
2. additionThe reaction medium is mixed for 90 minutes
3. customThe product of the coupling reaction
4. washis washed 3 times with DCM, 1 time with methanol and 5 times with DMF
5. additiona mixture of 30% piperidine, 35% toluene and 35% DMF
6. customThe product obtained from the deprotection
7. washThis product is washed 5 times with DMF, 1 time with methanol and 3 times with DCM