反应详情

To a 1-L 3-neck round bottom flask equipped with a magnetic stirrer, nitrogen inlet and thermocouple is added (2S)-2-[(tert-butoxycarbonyl)amino]-3-(3,5-difluorophenyl)propanoic acid (I, 40 g, 0.133 moles, 1 equivalent) followed by THF (240 mL). Lithium hydroxide monohydrate (5.6 g, 0.133 moles, 1 equivalent) is added in a single portion and is allowed to stir for 30 min at which time, the contents are cooled to 0°. Once cooled, dimethyl sulfate (12.6 mL, 0.133 moles, 1 equivalent) is added dropwise via syringe and then stirred for 30 min. The mixture is then heated to about 50° and monitored (by HPLC) until 90% conversion had been achieved. At that time, the mixture is cooled to below 20° (solids form). The mixture is then poured into sodium bicarbonate (200 mL), stirred for 15 min then extracted with methyl t-butyl ether (200 mL). The phases are separated and the aqueous layer is extracted with methyl t-butyl ether (2×200 mL). The combined organic phases are washed with water (400 mL) dried over sodium sulfate, filtered and concentrated under reduced pressure to give a solid. This material is then recrystallized from hexanes to give the title compound, NMR (DMSO-d6) δ 7.51, 7.15–7.25, 4.43, 3.81, 3.00–3.26 and 1.49; CMR (DMSO-d6) δ 172.43, 163.74, 161.20, 155.67, 142.58, 112.70, 120.23, 78.69, 54.71, 52.24, 39.25 and 28.37.

1. customTo a 1-L 3-neck round bottom flask equipped with a magnetic stirrer, nitrogen inlet
2. temperaturethe contents are cooled to 0°
3. temperatureOnce cooled
4. stirringstirred for 30 min
5. temperatureThe mixture is then heated to about 50°
6. temperatureAt that time, the mixture is cooled to below 20° (solids form)
7. stirringstirred for 15 min
8. extractionthen extracted with methyl t-butyl ether (200 mL)
9. customThe phases are separated
10. extractionthe aqueous layer is extracted with methyl t-butyl ether (2×200 mL)
11. washThe combined organic phases are washed with water (400 mL)
12. dry with materialdried over sodium sulfate
13. filtrationfiltered
14. concentrationconcentrated under reduced pressure
15. customto give a solid
16. customThis material is then recrystallized from hexanes