Sodium Chloride → L-Glutamic Acid

HRID 1296345Notes

Reaction equation

HRID 1296345 reaction equation
O=C[C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])O)O)O)[OH:4].S([O-])([O-])(=O)=O.[NH4+:18].[NH4+].[OH2:20].[OH2:21].O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].P([O-])(O)(O)=O.[K+].[Cl-].[Na+].O.O.O.O.O.O.O.[Cl-].[Ca+2].[Cl-].B(O)(O)O>O.O.O.O.S([O-])([O-])(=O)=O.[Mn+2].O.O.S([O-])([O-])(=O)=O.[Zn+2].O.O.O.O.O.S([O-])([O-])(=O)=O.[Cu+2].O.O.O.O.O.O.[Co](Cl)Cl.O.O.[O-][Mo]([O-])(=O)=O.[Na+].[Na+]>[NH2:18][C@H:5]([C:3]([OH:4])=[O:21])[CH2:7][CH2:9][C:11]([OH:12])=[O:20]

Participants

13

Reactant 7

HCID 5234Sodium ChlorideHCID 7628Boric AcidHCID 24843Magnesium Sulfate HeptahydrateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphateHCID 6097028Ammonium SulfateHCID 42636034Calcium;dichloride;heptahydrate

Product 1

HCID 33032L-Glutamic Acid

Reagents, catalysts & solvents 5

HCID 24463Copper sulfate pentahydrateCatalystHCID 517166Dichlorocobalt hexahydrateCatalystHCID 3067215Zinc sulfate, dihydrateCatalystHCID 16211258Sodium molybdate dihydrateCatalystHCID 22572548Manganese sulfate tetrahydrateCatalyst

Procedure

The strains Enterobacter agglomerans AJ13355 and the Klebsiella planticola AJ13399 were transformed with pMWC or pMWCB. Each of the resulting transformants AJ13355/pMWC, AJ13355/pMWCB, AJ13399/pMWC and AJ13399/pMWCB and the parent strains were inoculated into 500 ml-volume flask containing 20 ml of culture medium comprising 40 g/L glucose, 20 g/L ammonium sulfate, 0.5 g/L magnesium sulfate heptahydrate, 2 g/L potassium dihydrogenphosphate, 0.5 g/L sodium chloride, 0.25 g/L calcium chloride heptahydrate, 0.02 g/L ferrous sulfate heptahydrate, 0.02 g/L manganese sulfate tetrahydrate, 0.72 mg/L zinc sulfate dihydrate, 0.64 mg/L copper sulfate pentahydrate, 0.72 mg/L cobalt chloride hexahydrate, 0.4 mg/L boric acid, 1.2 mg/L sodium molybdate dihydrate, 2 g/L yeast extract, and 30 g/L calcium carbonate, and cultured at 37° C. for 15 hours with shaking. After the cultivation was completed, L-glutamic acid accumulated in the culture medium and residual glucose were measured. The results are shown in Table 1.

Workup

  1. additioncontaining 20 ml of culture medium
  2. extractionextract

Sources & evidence

ORD record
ord-9201329fab2e47b39a76ef09629ade86
Source dataset
uspto-grants-2007_07
Patent number
US07247459B1

Reaction notes

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