Sodium Fluoride → Tromethamine hydrochloride
Reaction equation
[Mg+2].[Cl-:2].[Cl-].SC[C@H]([C@@H:8](CS)[OH:9])O.P(OC[C@H]1[O:30][C@@H:29](N2C3N=CN=C(N)C=3N=C2)[C@H:28](O)[C@@H:27]1[OH:42])(OP(OP(O)(O)=O)(O)=O)(=O)O.[F-].[Na+].C[C@@]1([N:54]2C=C(C)C(=O)NC2=O)O[C@H](CO)[C@@H](O)C1>>[CH2:8]([OH:9])[C:28]([NH2:54])([CH2:27][OH:42])[CH2:29][OH:30].[ClH:2]Participants
Procedure
The competion experiments were carried out in the following way: The standard reaction mixture contained 2.5 mM MgCl2, 10 mM dithio threitol, 1 mg/ml bovine serum albumin, 2.5 mM ATP, 10 mM NaF, 2 μM methyl-3H-thymidine, an appropriate amount of a nucleoside analog and the multisubstrate deoxyribonucleoside kinase in an amount resulting in a linear conversion of the substrate, in a total reaction mixture of 50 μl of 50 mM Tris-HCl, pH 8.0. The reaction mixture was incubated at 37° C. for 30 min, and the reaction was terminated by spotting on to DE-81 discs (Whatman). The discs were instantly immersed and washed in ethanol (70%). The filters were dried and assayed for radioactivity in a tuluene-based scintillant. The concentration resulting in 50% inhibition of phosphorylation of 2 μM deoxythymidine was determined as a mean value of three independent experiments.
Workup
- customThe standard reaction mixture
Sources & evidence
- ORD record
- ord-f547fe2247df4c4db9c9f8cad3bd0304
- Source dataset
- uspto-grants-2003_06
- Patent number
- US06582947B1
Reaction notes
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