Trifluoroacetic acid → HCID 73442854, compound

Temperature

90 °C

A reaction mixture containing CuI (43.57 mg, 0.2288 mmol), Cs2CO3 (149.1 mg, 0.4576 mmol), 2-acetylcyclohexanone (64.15 mg, 0.4576 mmol), tert-butyl 2-(aminomethyl)pyrrolidine-1-carboxylate (137.5 mg, 0.6864 mmol) and 3-(3-(4-iodophenyl)isoxazol-5-yl)-5-(4-(isopropylsulfonyl)phenyl)pyrazin-2-amine (125 mg, 0.2288 mmol) in DMF (7.500 mL) was heated at 90° C. overnight. The reaction mixture was cooled to rt and partitioned between ethyl acetate (5 mL) and water (5 mL) and the layers separated. The aqueous layer was extracted further with ethyl acetate (2×5 mL) and the combined organic extracts were washed sequentially with water (1×5 mL) and brine (1×5 mL), dried over MgSO4 and concentrated in vacuo which yielded an oil. The oil was purified by reverse phase preparative HPLC [Waters Sunfire C18, 100 Å column, 5 M, 19 mm×150 mm, (solvent A: 0.05% TFA in water; solvent B: 0.05% TFA in CH3CN) over 16 minutes at 20 mL/min]. The product fractions were combined and concentrated to leave a yellow solid. The solid was dissolved in CH2Cl2(10 mL) and TFA (2 mL) was added and the resulting solution stirred at rt for 5 h. The reaction mixture was concentrated in vacuo and the residue partitioned between CH2Cl2 (5 mL) and water (5 mL) and the combined organic extracts were dried over MgSO4 and concentrated in vacuo which was purified by reverse phase preparative HPLC [Waters Sunfire C18, 100 Å column, 5 M, 19 mm×150 mm, (solvent A: 0.05% TFA in water; solvent B: 0.05% TFA in CH3CN) over 16 minutes at 20 mL/min]. The product fractions were combined and concentrated to leave the title compound as a yellow solid (25 mg, 15% yield); 1H NMR (400.0 MHz, DMSO) d 1.19 (d, J=6.9 Hz, 6H), 1.68 (dd, J=8.2, 12.7 Hz, 1H), 1.93 (m, 2H), 2.15-2.11 (m, 1H), 3.23 (m, 2H), 3.45-3.39 (m, 1H), 3.56 (m, 1H), 3.71 (m, 2H), 6.80 (d, J=8.7 Hz, 2H), 7.18 (s, 1H), 7.65 (s, 1H), 7.80 (d, J=8.7 Hz, 2H), 7.94 (d, J=8.5 Hz, 2H), 8.38 (d, J=8.5 Hz, 2H), 8.47 (bs, 1H) and 8.94 (s, 2H) ppm; MS (ES+) 519.

1. temperatureThe reaction mixture was cooled to rt
2. custompartitioned between ethyl acetate (5 mL) and water (5 mL)
3. customthe layers separated
4. extractionThe aqueous layer was extracted further with ethyl acetate (2×5 mL)
5. washthe combined organic extracts were washed sequentially with water (1×5 mL) and brine (1×5 mL)
6. dry with materialdried over MgSO4
7. concentrationconcentrated in vacuo which
8. customyielded an oil
9. customThe oil was purified by reverse phase preparative HPLC [Waters Sunfire C18, 100 Å column, 5 M, 19 mm×150 mm, (solvent A: 0.05% TFA in water; solvent B: 0.05% TFA in CH3CN) over 16 minutes at 20 mL/min]
10. concentrationconcentrated
11. customto leave a yellow solid
12. concentrationThe reaction mixture was concentrated in vacuo
13. customthe residue partitioned between CH2Cl2 (5 mL) and water (5 mL)
14. dry with materialthe combined organic extracts were dried over MgSO4
15. concentrationconcentrated in vacuo which
16. customwas purified by reverse phase preparative HPLC [Waters Sunfire C18, 100 Å column, 5 M, 19 mm×150 mm, (solvent A: 0.05% TFA in water; solvent B: 0.05% TFA in CH3CN) over 16 minutes at 20 mL/min]
17. concentrationconcentrated

ORD record

ord-45db5e1a5cf344f88693993980448d19

Source dataset

uspto-grants-2014_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08912198B2