Triethylamine → HCID 136333943, compound

To a solution of (1R*,3S,4R*,5S)-2-(tert-butoxycarbonyl)-5-hydroxy-2-azabicyclo[2.2.1]heptane-3-carboxylic acid (349 mg) and triethylamine (0.190 mL) in tetrahydrofuran (4 mL) was added 2-methylpropyl chlorocarbonate (0.178 mL) at 0° C., and the mixture was stirred at room temperature for 30 min. To the reaction system was added a solution of 3-amino-5-bromothiophene-2-carboxamide (200 mg) produced in Example 1, step D, in tetrahydrofuran (2 mL), and the mixture was stirred at 60° C. for 18 hr. Water was added to the reaction mixture, and the separated aqueous layer was extracted with ethyl acetate. The organic layer was washed with brine, and dried over anhydrous sodium sulfate. Insoluble material was filtered off, the filtrate was concentrated under reduced pressure, and the residue was purified by basic silica gel column chromatography (ethyl acetate/hexane). To the obtained pale-yellow amorphous solid (307 mg) were added 2M aqueous sodium hydroxide solution (1.81 mL) and ethanol (4.0 mL), and the mixture was stirred at 70° C. for 5 hr. The reaction system was neutralized with 1M hydrochloric acid at 0° C. To the reaction system was added water (2 mL), and the mixture was extracted with ethyl acetate. The organic layer was washed with brine, and dried over anhydrous sodium sulfate. Insoluble material was filtered off, and the filtrate was concentrated under reduced pressure. The residue was washed with ethyl acetate (2 mL) to give the title compound (211 mg) as a colorless solid.

1. extractionthe separated aqueous layer was extracted with ethyl acetate
2. washThe organic layer was washed with brine
3. dry with materialdried over anhydrous sodium sulfate
4. filtrationInsoluble material was filtered off
5. concentrationthe filtrate was concentrated under reduced pressure
6. customthe residue was purified by basic silica gel column chromatography (ethyl acetate/hexane)
7. additionTo the obtained pale-yellow amorphous solid (307 mg) were added 2M aqueous sodium hydroxide solution (1.81 mL) and ethanol (4.0 mL)
8. stirringthe mixture was stirred at 70° C. for 5 hr
9. customwas neutralized with 1M hydrochloric acid at 0° C
10. additionTo the reaction system was added water (2 mL)
11. extractionthe mixture was extracted with ethyl acetate
12. washThe organic layer was washed with brine
13. dry with materialdried over anhydrous sodium sulfate
14. filtrationInsoluble material was filtered off
15. concentrationthe filtrate was concentrated under reduced pressure
16. washThe residue was washed with ethyl acetate (2 mL)

ORD record

ord-c885ad13d9ef41fdbf061ee7a7e02042

Source dataset

uspto-grants-2014_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08921354B2