Diethylamine → HCID 86620332, compound

Temperature

0 °C

2-{[2-(Hydroxy)ethyl]sulfonyl}ethyl N,N,N′,N′-tetrakis(2-chloroethyl)phosphorodiamidate (150 mg, 0.31 mmol) was dissolved in dichloromethane (2 mL) and cooled to 0° C., and N,N-diisopropylethylamine (108 μL, 0.62 mmol) and trifluoromethanesulfonic anhydride (55 μL, 0.33 mmol) were added, and the reaction mixture allowed to stand for 15 minutes. The reaction mixture was added to a solution of diethylamine (64 μL, 0.62 mmol) in isopropyl acetate (2 mL), and this was allowed to stand for 30 minutes. The reaction mixture was diluted with isopropyl acetate (10 mL) and washed with 5% aqueous NaHCO3 (2×10 mL) and 1M aqueous HCl (2×5 mL). The aqueous acidic fractions were combined, washed with dichloromethane (10 mL), and solid NaHCO3 was added to pH>9. The mixture was extracted with isopropyl acetate (2×10 mL), and the isopropyl acetate extracts combined, dried over MgSO4, filtered, diluted with HCl (4.0M in dioxane, 1 mL), and concentrated under vacuum to give 2-{[2-(diethylamino)ethyl]sulfonyl}ethyl N,N,N′,N′-tetrakis(2-chloroethyl)phosphorodiamidate hydrochloride as a pale brown oil (32 mg, 19% yield). MS (ES+): m/z=536 [C16H34Cl4N3O4PS+H].

1. waitto stand for 30 minutes
2. washwashed with 5% aqueous NaHCO3 (2×10 mL) and 1M aqueous HCl (2×5 mL)
3. washwashed with dichloromethane (10 mL), and solid NaHCO3
4. additionwas added to pH>9
5. extractionThe mixture was extracted with isopropyl acetate (2×10 mL)
6. dry with materialdried over MgSO4
7. filtrationfiltered
8. additiondiluted with HCl (4.0M in dioxane, 1 mL)
9. concentrationconcentrated under vacuum

ORD record

ord-f5fd6a858d5a4b13a80f40a0ed54f81d

Source dataset

uspto-grants-2010_02

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07655799B2