D-Arabinose → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
Reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([CH2:9][OH:10])[OH:8])[OH:6])[OH:4].[O:11]=[CH:12][C@@H]([C@H]([C@H](CO)O)O)O.P(OC[C@H](O)[C@H](O)C(=O)CO)(O)(O)=O.O=C[C@H]([C@@H]([C@@H](CO)O)O)O>C(O)C>[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:12][OH:11])[OH:10])[OH:8])[OH:6])[OH:4]Participants
Reactant 4
Product 1
Reagents, catalysts & solvents 1
Procedure
The results presented in FIG. 3 show that in contrast to the control strain containing the shuttle vector alone (206C [pZB186]), the recombinant strain containing the added xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase genes demonstrated growth on and ethanol production from xylose and arabinose, alone or in a combination as a carbon source. The recombinant strain produces ethanol from glucose as efficiently as the control strain at 94% of theoretical yield. The recombinant strain additionally produces ethanol from xylose, or arabinose, or xylose and arabinose at process yields of 91%, 55%, and 79% yield in 96 hours (47 hours for xylose only), respectively. Furthermore, in the presence of glucose and xylose and arabinose, the recombinant strain ferments all three sugars to ethanol at a process yield of 79% yield within 48 hours, thus providing the foundation for advanced process designs with cofermentation of mixed-sugar feedstocks. The recombinant strain produces ethanol from 2% xylose, 2% arabinose, 2% xylose and 2% arabinose, or 1% glucose, 2% xylose and 2% arabinose at 92%, 88%, 94% or 90% of theoretical consumed sugar yield, respectively. The control strain neither grew on nor produced ethanol from xylose, arabinose or xylose and arabinose.
Sources & evidence
- ORD record
- ord-6f1c0cdea38648658eb33701a56b4b13
- Source dataset
- uspto-grants-1998_12
- Patent number
- US05843760
Reaction notes
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