4-Morpholineethanesulfonic acid → L-Hydroxyproline

HRID 1459129Notes

Reaction equation

HRID 1459129 reaction equation
C1N(CCS(O)(=O)=O)CC[O:3]C1.[NH:13]1[CH2:20][CH2:19][CH2:18][C@H:14]1[C:15]([OH:17])=[O:16].O=C(CCC([O-])=O)C([O-])=O.O=C1O[C@H]([C@H](CO)O)C(O)=C1O>>[OH:3][C@H:19]1[CH2:20][NH:13][C@H:14]([C:15]([OH:17])=[O:16])[CH2:18]1

Participants

5

Reactant 4

HCID 781654-Morpholineethanesulfonic acidHCID 145742L-ProlineHCID 164533Alpha-KetoglutarateHCID 54670067L-Ascorbic Acid

Product 1

HCID 5810L-Hydroxyproline

Procedure

The enzyme reaction with purified L-proline-4-hydroxylase obtained in Example 5 was carried out. The reaction mixture was composed of 200 mM MES buffer (pH 6.5), 20 mM L-proline, 20 mM 2-ketoglutarate, 5 mM L-ascorbic acid, 2 mM ferrous sulfates and 90 U of purified enzyme preparation in total volume of 50 μl. The reaction was carried out at 35° C. for 30 min. As a result of the reaction, 12.9 mM (1.7 g/l) of trans-4-hydroxyL-proline was produced in the reaction mixture.

Workup

  1. customobtained in Example 5
  2. customAs a result of the reaction

Sources & evidence

ORD record
ord-2aafba1825a74256874cc91267f22a2d
Source dataset
uspto-grants-1998_12
Patent number
US05854040

Reaction notes

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