反应详情

2.7 g (10 mmoles) of Boc-Gly-Pro-OH and 4.5 g (10 mmoles) of H-Glu(O-But)-Gly-OMe.TosOH are dissolved in 20 ml of dimethylformamide. 1.28 ml (10 mmoles) of N-ethylmorpholine and 2.2 g (10.6 mmoles) of dicyclohexylcarbodiimide are added and the mixture is left to stand at room temperature overnight. The dicyclohexylurea is then filtered off, the solvent is distilled off in vacuo, the residue is taken up in ethyl acetate and the solution is washed with citric acid, bicarbonate solution and water, as described above, dried over sodium sulfate and evaporated to dryness. The residue is dissolved in ether and a precipitate is obtained with petroleum ether and filtered off.

1. waitthe mixture is left
2. filtrationThe dicyclohexylurea is then filtered off
3. distillationthe solvent is distilled off in vacuo
4. washthe solution is washed with citric acid, bicarbonate solution and water
5. dry with materialdried over sodium sulfate
6. customevaporated to dryness
7. dissolutionThe residue is dissolved in ether
8. customa precipitate is obtained with petroleum ether
9. filtrationfiltered off