Bromophenol Blue → Acrylamide
Reaction equation
C(O)C([NH2:7])(CO)CO.Cl.CCCCCCCCC[CH2:19][CH2:20][CH2:21][O:22][S:23]([O-:26])(=[O:25])=[O:24].[Na+].C(S)C(O)C([OH:33])CS.C1C=CC2[S:44](=[O:46])(=[O:45])[O:43]C(C3C=C(Br)C(O)=C(Br)C=3)(C3C=C(Br)C(O)=C(Br)C=3)C=2C=1>>[C:21]([NH2:7])(=[O:22])[CH:20]=[CH2:19].[S:23]([O:22][O:43][S:44]([O-:46])(=[O:33])=[O:45])([O-:26])(=[O:25])=[O:24].[NH4+:7].[NH4+:7]Participants
Procedure
Standard cylinders of 10% acrylamide gel (9.7% acrylamide and 0.3% N,N'-methylene-bis-acrylogo there merized with TEMED) and ammonium persulfate were prepared and set up between gel and reservoir buffers comprising tris hydrochloride at pH 8.0 and 0.1% SDS. The upper gel surface was loaded with a charge comprising 50 micrograms of T2 pilus crystals, 20 ug of Clelands Reagent (0.01M) 1 mg. of SDS 20λ of glycol and 20λ of Bromophenol Blue (0.002%). Prior to charge the pili were heated with the SDS and the Cleland's reagent for 2 minutes at 100° C. The electrophoresis was run at 5 ma (at ca. 170 v) until the Bromophenol Blue had run 6 cm. The gels were removed and cut thru the dye band and two gels stained with Coomassie Blue Stain (0.2%) to give two bands--a major band and a minor band.
Workup
- additionPrior to charge the pili
- customThe gels were removed
- customto give two bands
Sources & evidence
- ORD record
- ord-191677b9554e4def974e1b414ebc9637
- Source dataset
- uspto-grants-1984_07
- Patent number
- US04461838
Reaction notes
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