反应详情

To a solution of (S)-2-[(5-cyclopropyl-6-cyclopropylmethoxy-pyrazine-2-carbonyl)-amino]-3,3-dimethyl-butyric acid methyl ester (58 mg, 160 μmol) in THF (3 mL) was added lithium hydroxide (30 mg, 715 μmol) in water (1 mL) and the mixture was stirred at reflux temperature for 3 hours. After cooling the mixture was poured into water (10 mL), acidified with 1 N HCl (1 mL) and extracted with TBME. Organic phases were combined, dried with Na2SO4, filtered and concentrated in vacuo to give the title compound (61 mg, quant.) as white solid; LC-MS (UV peak area/ESI) 100%, 348.1920 (M+H).

1. temperatureat reflux temperature for 3 hours
2. temperatureAfter cooling the mixture
3. extractionextracted with TBME
4. dry with materialdried with Na2SO4
5. filtrationfiltered
6. concentrationconcentrated in vacuo