反应详情

(S)-2-(3-(9H-Fluoren-9-yl)-N-methylpropanamido)-3-(tert-butoxy)propanoic acid (Compound SP745) (4.35 g, 11.0 mmol) and diisopropylethylamine (EtN(iPr)2) (10.4 mL, 60.0 mmol) were dissolved in dehydrated dichloromethane (46 ml), to the solution above, 2-chlorotrityl chloride resin (100-200 mesh, 1% DVB, purchased from Watanabe Chemical Industries, 4.65 g, 7.30 mmol) was added, and the amino acid was supported on the resin by shaking at room temperature for 60 minutes. The reaction solution was removed, and the resin was washed with dehydrated dichloromethane (46 ml) four times. A 20% solution of piperidine in N,N-dimethylformamide (15 ml) was added to the resin, and the Fmoc group was deprotected by shaking for 90 minutes. The reaction solution was removed, and the resin was washed with N,N-dimethylformamide (15 ml) three times. Subsequently, pent-4-enoic acid (2.3 ml, 21.9 mmol), 3H-(1,2,3)triazolo(4,5-b)pyridin-3-ol (HOAt) (1.99 g, 14.6 mmol) and N,N′-methanediylidenebis(propan-2-amine) (DIC, 3.71 ml, 24.1 mmol) were dissolved in N,N-dimethylformamide (15 ml), the solution was added to the resin, and the mixture was shaken at room temperature for 60 minutes for pentenoylation. The reaction solution was removed, and the resin was washed with N,N-dimethylformamide (15 ml) three times and then further washed with dichloromethane (15 ml) three times. Subsequently, a 1% solution of trifluoroacetic acid in dichloromethane (1% TFA in CH2Cl2) (40 ml) was added to the aforementioned resin, and the amino acid was cleaved from the resin by shaking for 30 minutes. The reaction solution was collected, and the resin was washed with dichloromethane (40 ml) three times. The collected reaction solution was concentrated under reduced pressure, and the residue was purified by reverse-phase silica gel column chromatography (10 mM aqueous ammonium acetate solution/methanol solution) to afford (S)-3-(tert-butoxy)-2-(N-methylpent-4-enamido)propanoic acid (Compound SP746) (1.02 g, 54%). Dichloromethane and N,N-dimethylformamide used for this synthesis were special grade solvents for peptide synthesis. (purchased from Watanabe Chemical Industries).

1. additionwas added
2. customThe reaction solution was removed
3. washthe resin was washed with dehydrated dichloromethane (46 ml) four times
4. additionA 20% solution of piperidine in N,N-dimethylformamide (15 ml) was added to the resin
5. stirringby shaking for 90 minutes
6. customThe reaction solution was removed
7. washthe resin was washed with N,N-dimethylformamide (15 ml) three times
8. additionthe solution was added to the resin
9. stirringthe mixture was shaken at room temperature for 60 minutes for pentenoylation
10. customThe reaction solution was removed
11. washthe resin was washed with N,N-dimethylformamide (15 ml) three times
12. washfurther washed with dichloromethane (15 ml) three times
13. additionSubsequently, a 1% solution of trifluoroacetic acid in dichloromethane (1% TFA in CH2Cl2) (40 ml) was added to the aforementioned resin
14. stirringby shaking for 30 minutes
15. customThe reaction solution was collected
16. washthe resin was washed with dichloromethane (40 ml) three times
17. customThe collected reaction solution
18. concentrationwas concentrated under reduced pressure
19. customthe residue was purified by reverse-phase silica gel column chromatography (10 mM aqueous ammonium acetate solution/methanol solution)