反应详情

200 μl of a 0.05-molar solution of 2-[(5-chloro-thiophene-2-carbonyl)-amino]-propionic acid, which additionally contains 0.05 mol/l DIPEA, in DMF are combined with 100 μl of a 0.1-molar solution of HATU in DMF on a microtitre plate and mixed for 10 min on a shaker. Then 200 μl of a 0.05-molar solution of 4-(4-ethyl-piperazin-1-yl-carbonyl)-aniline which additionally contains 0.05 mol/l DIPEA are pipetted in and the mixture is again combined by jogging. After 16 h at 80° C. the mixture is filtered through basic aluminium oxide and washed again with DMF/methanol=9:1. Then it is evaporated down i. vac.

1. additionmixed for 10 min on a shaker
2. filtrationAfter 16 h at 80° C. the mixture is filtered through basic aluminium oxide
3. washwashed again with DMF/methanol=9:1
4. customThen it is evaporated down i