反应详情

温度

120 °C

A mixture of 8-bromo-2-(2-isopropyl-5-methyl-2H-[1,2,4]triazol-3-yl)-4,5-dihydro-6-oxa-1,3a-diazabenzo[e]azulene (150 mg, 0.386 mmol), 1-isopropylpiperidine-4-thiol (92 mg, 0.579 mmol), Pd2(dba)3 (18 mg, 5 mol %), XantPhos (23 mg, 10 mol %) and DIPEA (0.27 mL, 1.54 mmol) in dioxane (4 mL) was purged with nitrogen and then heated at 120° C. for 1 h using microwave irradiation. After cooling to RT, the crude mixture was diluted with DCM (75 mL) and purified by column chromatography (Si-PCC, gradient 0-6% 2N NH3/MeOH in DCM followed by C18, gradient 20-50% MeOH in 0.5% TFA/H2O). The product containing fractions were combined and concentrated in vacuo. The resulting residue was loaded onto an Isolute® SCX-2 cartridge which was washed with MeOH and the product eluted with 0.5M NH3/MeOH. The basic fractions were concentrated under reduced pressure affording 2-(2-Isopropyl-5-methyl-2H-[1,2,4]triazol-3-yl)-8-(1-isopropylpiperidin-4-ylsulfanyl)-4,5-dihydro-6-oxa-1,3a-diazabenzo[e]azulene as a colorless solid (127 mg, 70%). LCMS: RT 3.00 min [M+H]+ 467.1

1. customwas purged with nitrogen
2. custommicrowave irradiation
3. temperatureAfter cooling to RT
4. additionthe crude mixture was diluted with DCM (75 mL)
5. custompurified by column chromatography (Si-PCC, gradient 0-6% 2N NH3/MeOH in DCM followed by C18, gradient 20-50% MeOH in 0.5% TFA/H2O)
6. additionThe product containing fractions
7. concentrationconcentrated in vacuo
8. washwas washed with MeOH
9. washthe product eluted with 0.5M NH3/MeOH
10. concentrationThe basic fractions were concentrated under reduced pressure