5'-Atp → L-Tryptophan

HRID 1588002Notes

Reaction equation

HRID 1588002 reaction equation
C(O)[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3]O.Cl.C(S)[C@@H](O)[C@H]([OH:15])CS.[Mg+2].[Cl-].[Cl-].P(O[CH2:34][C@H:35]1O[C@@H:38]([N:40]2[C:49]3N=CN=[C:44](N)[C:43]=3N=C2)[C@H:37](O)[C@@H:36]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>>[NH2:7][C@H:2]([C:5]([OH:15])=[O:6])[CH2:3][C:37]1[C:36]2[C:49](=[CH:43][CH:44]=[CH:34][CH:35]=2)[NH:40][CH:38]=1

Participants

5

Reactant 4

HCID 59575'-Atp2×HCID 93573Tromethamine hydrochloride2×HCID 446094D-1,4-Dithiothreitol2×HCID 5360315Magnesium Chloride2×

Product 1

HCID 6305L-Tryptophan

Procedure

The ten oligodeoxynucleotides shown in FIG. 14 were synthesized by the phosphotriester method and purified. 500 pmole of each oligodeoxynucleotide except 1 and 10 were phosphorylated individually in 20 ul containing 60 mM Tris-HCl, pH 8, 15 mM DTT, 10 mM MgCl2, 20 uCi of [lambda-32P]-ATP and 20 units of polynucleotide kinase (P/L Biochemicals) for 30 min. at 37° C. This was followed by the addition of 10 ul containing 60 mM Tris-HCl, pH 8, 15 mM DTT, 10 mM MgCl2, 1.5 mM ATP and 20 additional units of polynucleotide kinase followed by another 30 min incubation at 37° C. Following incubation the samples were incubated at 100° C. for 5 min. 500 pmole of oligodeoxynucleotides 1 and 10 were diluted to 30 ul in the above buffer without ATP.

Workup

  1. custompurified
  2. additionThis was followed by the addition of 10 ul
  3. customat 37° C
  4. waitwere incubated at 100° C. for 5 min

Sources & evidence

ORD record
ord-e64a7b76a1a34cb8b915c2afa4e49b5d
Source dataset
uspto-grants-1993_06
Patent number
US05220013

Reaction notes

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