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HRID1605807

反应详情

EQUATION

反应方程式

HRID 1605807 的结构方程式

REACTANTS

反应物

2
结构：C
HCID297

Methane

CH4

结构：NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O.O=S(=O)(O)O
HCID441374

Kanamycin A sulfate

C18H38N4O15S

PRODUCTS

生成物

1
结构：NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
HCID6032

Kanamycin A

C18H36N4O11

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
溶剂HCID107526

未命名化合物

CONDITIONS

反应条件

温度
120 °C

PROCEDURE

实验过程

Simulated kanamycin culture liquid is prepared by adding 80 mg of kanamycin sulfate to 160 ml of glucose-bouillon medium which is sterilized by heating at 120° C for 15 minutes. This liquid is then passed through a column (diameter: 1.5 cm), wherein 2.7 g of the molecular sieving particle containing activated charcoal obtained in Example 1 (sample (a)) is packed, at a flow rate of 0.5 ml/minute. The column is then subjected to washing with water and further to elution with 0.1N HCl-methanol (1:1) solvent. Active fractions are combined and 72.8 mg of kanamycin chloride is recovered in very high purity (decoloration degree: 99%; purity: 98%) and high yield (recovery percentage: 91%).

WORKUP

后处理

  1. washto washing with water and further to elution with 0.1N HCl-methanol (1:1) solvent
  2. custom72.8 mg of kanamycin chloride is recovered in very high purity (decoloration degree: 99%; purity: 98%)
  3. customhigh yield (recovery percentage: 91%)