Sulfuric Acid → Glucose xylose

A 100 ml screw cap bottle having an exhaust line with a check valve attached to the lid was used for the fermentation test. 50 ml of SDX medium (6.7 g/l yeast nitrogen base without amino acids and nucleic acids, 30 g/l D-glucose, 20 g/l xylose) with liquid yeast suspension added to a final OD600 of 10 of the fermentation medium was prepared, and fermentation was performed at 30° C., 100 rpm. The culture liquid was sampled periodically, and the substrate (glucose and xylose) and products (ethanol, glycerol and xylitol) were analyzed by liquid chromatography. An HPX-87H column (Bio-Rad) was used at 60° C. as the liquid chromatography column, and a RID-10A refractive index detector (Shimadzu Mfg.) was used as the detector. 0.05% sulfuric acid solution was used for the mobile phase, which was supplied at a rate of 0.6 ml/min. FIG. 12 shows changes over time in the substrate concentrations and product concentrations in fermentation medium of each transformant. The fermentation tests were performed twice or more, and the averages are given.

1. customA 100 ml screw cap bottle
2. customwas prepared
3. customwas performed at 30° C.
4. aliquotThe culture liquid was sampled periodically
5. customwas used at 60° C. as the liquid chromatography column
6. concentrationin the substrate concentrations and product concentrations in fermentation medium of each transformant

ORD record

ord-b5c517e3171841e8b0d3159d073d3469

Source dataset

uspto-grants-2014_07

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08772012B2