反应详情

To a stirred solution of (S)-1-cyclopropyl-3-(4-(4-(3-methylmorpholino)-6,7-dihydro-5H-pyrrolo[3,4-d]pyrimidin-2-yl)phenyl)urea (Example 3) (100 mg, 0.25 mmol) and N-methylmorpholine (56 μL, 0.5 mmol) in DMF (5 ml, anhydrous) was added EDC (53 mg, 0.28 mmol), HOBT (38 mg, 0.28 mmol), and 4-amino-4-oxobutanoic acid (29.7 mg, 0.25 mmol). The reaction mixture was stirred at room temperature until analysis by LCMS deemed reaction complete. Solvent was then removed in vacuo. Purified by prep. HPLC (high pH) to afford a white solid (25.7 mg, 0.052 mmol, 21%).

1. customreaction complete
2. customSolvent was then removed in vacuo
3. customPurified by prep