反应详情

To the above resin bound Fmoc β-alanine was added 500 μL of a 20% solution of piperidine in DMF. After 30 min of shaking, the resin was drained and washed with 1 mL DMF containing 1-hydroxybenzotriazole (50 mg/mL) and DMF (2×1 mL). Then 200 μmol 4-formylbenzoic acid (30 mg) and 200 μmol HOBt (31 mg) dissolved in DMF (500 μL) were added to the resin followed by 200 μmol diisopropyl carbodiimide (DIC, 25.2 mg) dissolved in acetonitrile (500 μL). The mixture was shaken for 4 hours at 25° C. followed by filtration and washing of the resin with DMF (3×1 mL).

1. washwashed with 1 mL DMF
2. additioncontaining 1-hydroxybenzotriazole (50 mg/mL) and DMF (2×1 mL)
3. additionwere added to the resin
4. stirringThe mixture was shaken for 4 hours at 25° C.
5. filtrationfollowed by filtration
6. washwashing of the resin with DMF (3×1 mL)